Multimodal Control of Bacterial Gene Expression by Red and Blue Light

Title data

Meier, Stefanie S. M. ; Multamäki, Elina ; Ranzani, Américo Tavares ; Takala, Heikki ; Möglich, Andreas:
Multimodal Control of Bacterial Gene Expression by Red and Blue Light.
In: Braman, Jeffrey Carl (ed.): Synthetic Biology : Methods and Protocols. 2nd Edition. - New York, NY : Humana Press , 2024 . - pp. 463-477 . - (Methods in Molecular Biology ; 2760 )
ISBN 978-1-0716-3657-2
DOI: https://doi.org/10.1007/978-1-0716-3658-9_26

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Abstract in another language

By applying sensory photoreceptors, optogenetics realizes the light-dependent control of cellular events and state. Given reversibility, noninvasiveness, and exquisite spatiotemporal precision, optogenetic approaches enable innovative use cases in cell biology, synthetic biology, and biotechnology. In this chapter, we detail the implementation of the pREDusk, pREDawn, pCrepusculo, and pAurora optogenetic circuits for controlling bacterial gene expression by red and blue light, respectively. The protocols provided here guide the practical use and multiplexing of these circuits, thereby enabling graded protein production in bacteria at analytical and semi-preparative scales.