Degradable Cell-Adhesive Hybrid Hydrogels by Cross-Linking of Gelatin with Poly(2-isopropenyl-2-oxazoline)

Title data

Yu, Peitao ; Sedlačík, Tomáš ; Parmentier, Laurens ; Jerca, Florica Adriana ; Jerca, Valentin Victor ; Van Vlierberghe, Sandra ; Leiske, Meike N. ; Hoogenboom, Richard:
Degradable Cell-Adhesive Hybrid Hydrogels by Cross-Linking of Gelatin with Poly(2-isopropenyl-2-oxazoline).
In: Biomacromolecules. Vol. 25 (2024) Issue 8 . - pp. 5332-5342.
ISSN 1526-4602
DOI: https://doi.org/10.1021/acs.biomac.4c00743

Official URL:

Abstract in another language

This study focused on the cross-linking of poly(2-isopropenyl-2-oxazoline) (PiPOx) with gelatin to obtain strong, degradable hybrid hydrogels with good cell adhesion. The molecular weight and concentration of PiPOx and the PiPOx-to-gelatin ratio were varied to adjust the mechanical and swelling properties of the hybrid hydrogels. The swelling degree of PiPOx-gelatin hydrogels in water ranged between 1260 and 810%, with the corresponding Young’s compressive moduli ranging from 77 to 215 kPa. Rheological measurements demonstrated the mechanical stability of the hydrogels. The hydrogels exhibited substantial degradation in Dulbecco’s phosphate-buffered saline (DPBS) and cell culture medium within several weeks, indicating their degradability and responsiveness. The cell adhesion assay with primary human foreskin fibroblasts revealed the hybrid hydrogels are noncytotoxic and support cell attachment and proliferation. These strong hydrogels thus show excellent potential as biomedical cell scaffolds, combining the tunability and strength of PiPOx hydrogels with gelatin’s cell-interactive properties while the ester-containing cross-links provide tunable degradability.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Additional notes:	PMID: 39059021
Institutions of the University:	Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Junior Professor Sustainable and Functional Polymer Systems > Junior Professor Sustainable and Functional Polymer Systems - Juniorprof. Dr. Meike Leiske
Result of work at the UBT:	Yes
DDC Subjects:	500 Science > 540 Chemistry
Date Deposited:	31 Jan 2025 09:24
Last Modified:	31 Jan 2025 09:24
URI:	https://eref.uni-bayreuth.de/id/eprint/92261