Compact Micropatterned Chip Empowers Undisturbed and Programmable Drug Addition in High-Throughput Cell Screening

Title data

Zhao, Yuanyi ; Sun, Yingxue ; Xie, Xinjian ; Liang, Yujia ; Cavalcanti-Adam, Elisabetta Ada ; Feng, Wenqian:
Compact Micropatterned Chip Empowers Undisturbed and Programmable Drug Addition in High-Throughput Cell Screening.
In: Advanced Materials. Vol. 36 (2024) Issue 11 . - 2306814.
ISSN 1521-4095
DOI: https://doi.org/10.1002/adma.202306814

Official URL:

Abstract in another language

Simultaneously adding multiple drugs and other chemical reagents to individual droplets at specific time points presents a significant challenge, particularly when dealing with tiny droplets in high-throughput screening applications. In this study, a micropatterned polymer chip is developed as a miniaturized platform for light-induced programmable drug addition in cell-based screening. This chip incorporates a porous superhydrophobic polymer film with atom transfer radical polymerization reactivity, facilitating the efficient grafting of azobenzene methacrylate, a photoconformationally changeable group, onto the hydrophilic regions of polymer matrix at targeted locations and with precise densities. By employing light irradiation, the cyclodextrin-azobenzene host–guest complexes formed on the polymer chip can switch from an “associated” to a “dissociated” state, granting precise photochemical control over the supramolecular coding system and its surface patterning ability. Significantly, the exceptional spatial and temporal control offered by these chemical transitions empowers to utilize digital light processing systems for simultaneous regulation and release of cyclodextrin-bearing drugs across numerous droplets containing suspended or adhered cells. This approach minimizes mechanical disruption while achieving precise control over the timing of addition, dosage, and integration varieties of released drugs in high-throughput screening, all programmable to meet specific requirements.

Further data

Item Type:	Article in a journal
Refereed:	Yes
Keywords:	cell screening; drug release; host–guest interaction; photoswitching; superhydrophobic–hydrophilic pattern
Institutions of the University:	Faculties > Faculty of Engineering Science > Chair Cellular Biomechanics > Chair Cellular Biomechanics - Univ.-Prof. Dr. Dr. Elisabetta Ada Cavalcanti-Adam
Result of work at the UBT:	Yes
DDC Subjects:	600 Technology, medicine, applied sciences
Date Deposited:	30 Oct 2025 13:26
Last Modified:	30 Oct 2025 13:26
URI:	https://eref.uni-bayreuth.de/id/eprint/95054