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Cell adhesion and proliferation on RGD-modified recombinant spider silk proteins

Title data

Wohlrab, Stefanie ; Müller-Herrmann, Susanne ; Schmidt, Andreas ; Neubauer, Stefanie ; Kessler, Horst ; Leal-Egaña, Aldo ; Scheibel, Thomas:
Cell adhesion and proliferation on RGD-modified recombinant spider silk proteins.
In: Biomaterials. Vol. 33 (October 2012) Issue 28 . - pp. 6550-6559.
ISSN 1878-5905
DOI: https://doi.org/10.1016/j.biomaterials.2012.05.069

Official URL: Volltext

Abstract in another language

Due to the biocompatibility and biodegradability as well as the mechanical properties of the fibers, spider silk has become an attractive material for researchers regarding biomedical applications. In this study, the engineered recombinant spider silk protein eADF4(C16) was modified with the integrin recognition sequence RGD by a genetic (fusing the amino acid sequence GRGDSPG) as well as a chemical approach (using the cyclic peptide c(RGDfK)). Both modified silk proteins were processed into films, and thereafter characterized concerning secondary structure, water contact angle and surface roughness.
No influence of the RGD-modifications on any of these film properties could be detected. However, attachment and proliferation of BALB/3T3 mouse fibroblasts were significantly improved on films made of the RGDmodified silk proteins. Interestingly, the genetically created hybrid protein (with a linear RGD sequence) showed similar or slightly better cell adhesion properties as the silk protein chemically modified with the cyclic RGD peptide.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: Artikel wurde zunächst am 22.06.2012 online veröffentlicht
Keywords: Silk; Genetic engineering; Chemical coupling; Cell adhesion; Cell proliferation
RGD peptide
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Emerging Fields > Food and Health Sciences
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Emerging Fields
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 24 Jun 2015 07:30
Last Modified: 09 Apr 2018 09:08
URI: https://eref.uni-bayreuth.de/id/eprint/15358