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A novel Dps-type protein from insect gut bacteria catalyses hydrolysis and synthesis of N-acyl amino acids

Title data

Ping, Liyan ; Büchler, Rita ; Mithöfer, Axel ; Svatoš, Aleš ; Spiteller, Dieter ; Dettner, Konrad ; Gmeiner, Sophie ; Piel, Jörn ; Schlott, Bernhard ; Boland, Wilhelm:
A novel Dps-type protein from insect gut bacteria catalyses hydrolysis and synthesis of N-acyl amino acids.
In: Environmental Microbiology. Vol. 9 (2007) Issue 6 . - pp. 1572-1583.
ISSN 1462-2920
DOI: https://doi.org/10.1111/j.1462-2920.2007.01279.x

Abstract in another language

A novel type of a microbial N-acyl amino acid hydrolase (AAH) from insect gut bacteria was purified, cloned and functionally characterized. The enzyme was obtained from Microbacterium arborescens SE14 isolated from the foregut of larvae of the generalist herbivore Spodoptera exigua. The substrates of AAH are N-acyl-glutamines previously reported to elicit plant defence reactions after introduction into the leaf during feeding. The isolated AAH catalyses the hydrolysis of the amide bond (Km = 36 μmol l-1) and, less efficient, the formation (Km = 3 mmol l-1) of the elicitor active N-acyl amino acids. The AAH from M. arborescens SE14 shows no homology to known fatty acyl amidases (EC 3.5.1.4) but belongs to the family of Dps proteins (DNA-binding protein from starved cell). In line with other DPS proteins AAH is a homododecamer (monomer 17 181 Da) and contains iron atoms (c. 1–16 iron atoms per subunit). Unlike genuine DPS proteins the enzyme does not significantly bind DNA. Amino acid hydrolase is the first member of the DPS family that catalyses the cleavage or formation of amide bonds. The participation of a microbial enzyme in the homeostasis of N-acyl-glutamines in the insect gut adds further complexity to the interaction between plants and their herbivores.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER50500
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Animal Ecology II - Evolutionary Animal Ecology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Former Professors > Chair Animal Ecology II - Univ.-Prof. Dr. Konrad Dettner
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Former Professors
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 29 Jun 2015 06:09
Last Modified: 29 Jun 2015 06:09
URI: https://eref.uni-bayreuth.de/id/eprint/15523