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Consumers of 4‐chloro‐2‐methylphenoxyacetic acid from agricultural soil and drilosphere harbor cadA, r/sdpA, and tfdA‐like gene encoding oxygenases

Title data

Liu, Ya-Jun ; Liu, Shuang-Jiang ; Drake, Harold L. ; Horn, Marcus A.:
Consumers of 4‐chloro‐2‐methylphenoxyacetic acid from agricultural soil and drilosphere harbor cadA, r/sdpA, and tfdA‐like gene encoding oxygenases.
In: FEMS Microbiology Ecology. Vol. 86 (2013) Issue 1 . - pp. 114-129.
ISSN 1574-6941
DOI: https://doi.org/10.1111/1574-6941.12144

Abstract in another language

Microbial degradation of 2-Methyl-4-chlorophenoxyacetic acid (MCPA) in soil is enhanced by earthworms and intiated by tfdA-like, cadA and r/sdpA gene encoded oxygenases. Copy numbers of such genes increased during MCPA degradation in soil, and MCPA stimulated transcription of tfdA-like and r/sdpA genes up to 4x. Transcription of cadA was detected with MCPA only. DNA stable isotope probing after consumption of 0.6-0.8 mg 13C-MCPA gdw-1 in oxic microcosms indicated diverse labeled oxygenase genes in bulk soil, burrow walls and cast. 9, 6 and 3 operational taxonomic units of tfdA-like, cadA and r/sdpA genes, respectively, were labeled, and affiliated with group 2 Alphaproteobacteria including Bradyrhizobia and group 1 class III Betaproteobacteria. New genes encoding putative MCPA oxygenases were identified. Diversity of labeled OTUs tended to be lower for cast than for bulk soil. The collective data indicate (i) hitherto unknown active MCPA degraders and/or oxygenase genes in soil, (ii) that multiple oxygenases are associated with MCPA degradation in soil at the same time, (iii) that earthworms impact the capability of MCPA degraders in soil to respond to MCPA, and (iv) enables a more in depth analysis of MCPA degrader communities in soil by future structural gene based experimental strategies.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER115399
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 22 Jul 2015 05:41
Last Modified: 22 Jul 2015 05:41
URI: https://eref.uni-bayreuth.de/id/eprint/16982