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Optimization of 14C liquid scintillation counting of plant and soil lipids to trace short term formation, translocation and degradation of lipids

Title data

Wiesenberg, Guido L. B. ; Gocke, Martina ; Kuzyakov, Yakov:
Optimization of 14C liquid scintillation counting of plant and soil lipids to trace short term formation, translocation and degradation of lipids.
In: Journal of Radioanalytical and Nuclear Chemistry. Vol. 284 (2010) Issue 1 . - pp. 99-108.
ISSN 1588-2780
DOI: https://doi.org/10.1007/s10967-010-0450-7

Abstract in another language

Two powerful approaches are frequently used to trace incorporation and degradation of plant derived C in soil: 14C labelling/chasing and analysis of lipid composition. In this study, we coupled these approaches in order to trace short term incorporation of plant derived lipids into rhizosphere and non-rhizosphere soil. Methodological optimization was required and implied 14C liquid scintillation counting improvement for plant lipid extracts taking into account organic solvents, solvent-to-scintillation cocktail ratio, and amount of lipids. Following method optimization, 14C data of fatty acids indicated a notable contribution of root derived lipids to rhizosphere and nonrhizosphere soil. Coupling of 14C labelling/chasing with lipid analysis is a powerful and cheap approach for tracing of root derived C in soil allowing for estimation of C budget, for determination of C formation and translocation within plants and from plant to soil, as well as for identification of short term dynamics of specific compoundclasses within soil.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER70815
Institutions of the University: Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Earth Sciences
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 22 Jul 2015 06:23
Last Modified: 22 Jul 2015 06:23
URI: https://eref.uni-bayreuth.de/id/eprint/17022