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Wiesenberg, Guido L. B. ; Gocke, Martina ; Kuzyakov, Yakov:
Optimization of 14C liquid scintillation counting of plant and soil lipids to trace short term formation, translocation and degradation of lipids.
In: Journal of Radioanalytical and Nuclear Chemistry.
Bd. 284
(2010)
Heft 1
.
- S. 99-108.
ISSN 1588-2780
DOI: https://doi.org/10.1007/s10967-010-0450-7
Abstract
Two powerful approaches are frequently used to trace incorporation and degradation of plant derived C in soil: 14C labelling/chasing and analysis of lipid composition. In this study, we coupled these approaches in order to trace short term incorporation of plant derived lipids into rhizosphere and non-rhizosphere soil. Methodological optimization was required and implied 14C liquid scintillation counting improvement for plant lipid extracts taking into account organic solvents, solvent-to-scintillation cocktail ratio, and amount of lipids. Following method optimization, 14C data of fatty acids indicated a notable contribution of root derived lipids to rhizosphere and nonrhizosphere soil. Coupling of 14C labelling/chasing with lipid analysis is a powerful and cheap approach for tracing of root derived C in soil allowing for estimation of C budget, for determination of C formation and translocation within plants and from plant to soil, as well as for identification of short term dynamics of specific compoundclasses within soil.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Zusätzliche Informationen: | BAYCEER70815 |
| Institutionen der Universität: | Forschungseinrichtungen > Forschungszentren > Bayreuther Zentrum für Ökologie und Umweltforschung - BayCEER Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Geowissenschaften Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Forschungseinrichtungen Forschungseinrichtungen > Forschungszentren |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik |
| Eingestellt am: | 22 Jul 2015 06:23 |
| Letzte Änderung: | 22 Jul 2015 06:23 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/17022 |