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Effect of earthworm feeding guild on ingested dissimilatory nitrate reducers and denitrifiers in the alimentary canal of the earthworm

Title data

Depkat-Jakob, Peter ; Hilgarth, Maik ; Horn, Marcus A. ; Drake, Harold L.:
Effect of earthworm feeding guild on ingested dissimilatory nitrate reducers and denitrifiers in the alimentary canal of the earthworm.
In: Applied and Environmental Microbiology. Vol. 76 (2010) Issue 18 . - pp. 6205-6214.
ISSN 1098-5336
DOI: https://doi.org/10.1128/AEM.01373-10

Abstract in another language

The earthworm gut is an anoxic nitrous oxide (N2O)-emitting microzone in aerated soils. In situ conditions of the gut might stimulate ingested nitrate-reducing soil bacteria linked to this emission. The objective of this study was to determine if dissimilatory nitrate reducers and denitrifiers in the alimentary canal were affected by feeding guilds (epigeic [Lumbricus rubellus], anecic [Lumbricus terrestris], and endogeic [Aporrectodea caliginosa]). Genes and gene transcripts of narG (encodes for a subunit of nitrate reductase and targets both dissimilatory nitrate reducers and denitrifiers) and nosZ (encodes for a subunit of N2O reductase and targets denitrifiers) were detected in guts and soils. Gut derived sequences were similar to those of cultured and uncultured soil bacteria, and also to soil derived sequences obtained in this study. Gut derived narG sequences and narG terminal restriction fragments (TRFs) were mainly affiliated with gram positive organisms (Actinobacteria). The majority of gut and uppermost soil derived narG transcripts affiliated with Mycobacterium (Actinobacteria). In contrast, narG sequences indicative of gram negatives (Proteobacteria) were dominant in mineral soil. Most nosZ sequences and nosZ TRFs were affiliated with Bradyrhizobium (Alphaproteobacteria) and uncultured soil bacteria. TRF profiles indicated that nosZ transcripts were more affected by earthworm feeding guilds than were nosZs, whereas narG transcripts were less affected by earthworm feeding guilds than were narGs. narG and nosZ transcripts were different and less diverse in the earthworm gut than in mineral soil. The collective results indicated that dissimilatory nitrate reducers and denitrifiers in the earthworm gut were soil derived and that ingested narG- and nosZ-containing taxa were not uniformly stimulated in the guts of worms from different feeding guilds

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER86431
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 29 Jul 2015 05:53
Last Modified: 29 Jul 2015 05:53
URI: https://eref.uni-bayreuth.de/id/eprint/17361