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High unique diversity of sulfate-reducing prokaryotes characterized in a depth gradient in an acidic fen

Title data

Schmalenberger, Achim ; Drake, Harold L. ; Küsel, Kirsten:
High unique diversity of sulfate-reducing prokaryotes characterized in a depth gradient in an acidic fen.
In: Environmental Microbiology. Vol. 9 (2007) Issue 5 . - pp. 1317-1328.
ISSN 1462-2920
DOI: https://doi.org/10.1111/j.1462-2920.2007.01251.x

Abstract in another language

The dissimilatory reduction of sulfate contributes to the retention of sulfur in acidic mineratrophic peatlands. Novel sulfate-reducing prokaryotes (SRPs) colonize these low-sulfate fens. This study assessed the community structures of SRPs in a depth gradient (0–50 cm) in a fen, located in the Fichtelgebirge (Spruce Mountains), Germany. Detection of SRPs with multiplex (terminal-) restriction fragment length polymorphism analysis of amplified dissimilatory (bi)sulfite reductase genes (dsrAB) separated three subgroups derived from (i) the upper 5 and 10 cm, (ii) 15–25 cm, and (iii) 30–50 cm depth. Biogeochemical parameters measured in the soil solution from July 2001 to July 2004 documented that the upper 5–10 cm were exposed to drying and oxygenation prior to sampling. Periodic oxygenation reached a maximum depth of 25 cm in the water-saturated fen and was concomitant with relative high concentrations of nitrate (120 mM) and sulfate (up to 310 mM). The fen soil was permanently anoxic below 30 cm depth with average concentrations of sulfate below 40 mM and maximum concentrations of methane. Cloning of dsrAB PCR products from 5, 20 and 40 cm depth yielded a total of 84 unique dsrAB restriction patterns. Partial sequencing of 61 distinct clones resulted in 59 unique partial protein sequences that mainly clustered with DsrA sequences of uncultivated sulfate reducers. Syntrophobacter fumaroxidans- and Syntrophobacter wolinii-related bacteria appeared to be present only in 40 cm depth. Differences in the SRP community structures suggested that SRPs present in the upper fen soil have to tolerate O2 and even drying, whereas SRPs present in deep anoxic zones may act as syntrophic fermentors in cooperation with H2-utilizing methanogens.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER37713
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 29 Jul 2015 05:53
Last Modified: 18 Sep 2015 08:56
URI: https://eref.uni-bayreuth.de/id/eprint/17399