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Ion and seed dependent fibril assembly of a spidroin core domain

Title data

Humenik, Martin ; Smith, Andrew M. ; Arndt, Sina ; Scheibel, Thomas:
Ion and seed dependent fibril assembly of a spidroin core domain.
In: Journal of Structural Biology. Vol. 191 (June 2015) Issue 2 . - pp. 130-138.
ISSN 1047-8477
DOI: https://doi.org/10.1016/j.jsb.2015.06.021

Abstract in another language

Recombinant eADF4(C16) represents an engineered spider silk variant based on the sequence of the core
domain of the natural dragline silk protein ADF4 of Araneus diadematus. Previously eADF4(C16) has been
shown to self-assemble into cross-b fibrils in a two-step process of nucleus formation and fibril growth.
Here, it is shown that structurally converted low molecular weight oligomers can act as nuclei. Further, it
could be determined that specifically potassium and phosphate ions strongly influence both nucleus
formation as well as fibril growth. Nucleation of fibril assembly could be surpassed by seeding soluble
protein with pre-assembled fibrils but also, unexpectedly, with eADF4(C16) sub-micrometer particles.
The latter finding reveals that spider silk fibril assembly seems to be rather dependent on the protein
sequence than on the structural features, since cross-seeding with other proteins was not possible.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Fibril Nucleus Particle Recombinant spider silk seed
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Emerging Fields > Food and Health Sciences
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Emerging Fields
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 21 Sep 2015 12:39
Last Modified: 20 May 2016 12:33
URI: https://eref.uni-bayreuth.de/id/eprint/19465