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Ion and seed dependent fibril assembly of a spidroin core domain

Titelangaben

Humenik, Martin ; Smith, Andrew M. ; Arndt, Sina ; Scheibel, Thomas:
Ion and seed dependent fibril assembly of a spidroin core domain.
In: Journal of Structural Biology. Bd. 191 (Juni 2015) Heft 2 . - S. 130-138.
ISSN 1047-8477
DOI: https://doi.org/10.1016/j.jsb.2015.06.021

Abstract

Recombinant eADF4(C16) represents an engineered spider silk variant based on the sequence of the core
domain of the natural dragline silk protein ADF4 of Araneus diadematus. Previously eADF4(C16) has been
shown to self-assemble into cross-b fibrils in a two-step process of nucleus formation and fibril growth.
Here, it is shown that structurally converted low molecular weight oligomers can act as nuclei. Further, it
could be determined that specifically potassium and phosphate ions strongly influence both nucleus
formation as well as fibril growth. Nucleation of fibril assembly could be surpassed by seeding soluble
protein with pre-assembled fibrils but also, unexpectedly, with eADF4(C16) sub-micrometer particles.
The latter finding reveals that spider silk fibril assembly seems to be rather dependent on the protein
sequence than on the structural features, since cross-seeding with other proteins was not possible.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: Fibril Nucleus Particle Recombinant spider silk seed
Institutionen der Universität: Fakultäten
Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien > Lehrstuhl Biomaterialien - Univ.-Prof. Dr. Thomas Scheibel
Profilfelder > Advanced Fields > Neue Materialien
Profilfelder > Advanced Fields > Molekulare Biowissenschaften
Profilfelder > Advanced Fields > Polymer- und Kolloidforschung
Profilfelder > Emerging Fields > Lebensmittel- und Gesundheitswissenschaften
Profilfelder
Profilfelder > Advanced Fields
Profilfelder > Emerging Fields
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 600 Technik, Medizin, angewandte Wissenschaften
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Eingestellt am: 21 Sep 2015 12:39
Letzte Änderung: 20 Mai 2016 12:33
URI: https://eref.uni-bayreuth.de/id/eprint/19465