bei Google ScholarTitelangaben
Pirzer, Tobias ; Geisler, Michael ; Scheibel, Thomas ; Hugel, Thorsten:
Single molecule force measurements delineate salt, pH and surface effects on biopolymer adhesion.
In: Physical Biology.
Bd. 6
(2009)
Heft 2
.
- No. 025004.
ISSN 1478-3975
DOI: https://doi.org/10.1088/1478-3975/6/2/025004
Abstract
In this paper we probe the influence of surface properties, pH and salt on the adhesion of recombinant spider silk proteins onto solid substrates with single molecule force spectroscopy. A single engineered spider silk protein (monomeric C(16) or dimeric (QAQ)(8)NR3) is covalently bound with one end to an AFM tip, which assures long-time measurements for hours with one and the same protein. The tip with the protein is brought into contact with various substrates at various buffer conditions and then retracted to desorb the protein. We observe a linear dependence of the adhesion force on the concentration of three selected salts (NaCl, NaH(2)PO(4) and NaI) and a Hofmeister series both for anions and cations. As expected, the more hydrophobic C(16) shows a higher adhesion force than (QAQ)(8)NR3, and the adhesion force rises with the hydrophobicity of the substrate. Unexpected is the magnitude of the dependences--we never observe a change of more than 30%, suggesting a surprisingly well-regulated balance between dispersive forces, water-structure-induced forces as well as co-solute-induced forces in biopolymer adhesion.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Institutionen der Universität: | Fakultäten Fakultäten > Fakultät für Ingenieurwissenschaften Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien > Lehrstuhl Biomaterialien - Univ.-Prof. Dr. Thomas Scheibel Profilfelder > Advanced Fields > Neue Materialien Profilfelder > Advanced Fields > Molekulare Biowissenschaften Profilfelder > Advanced Fields > Polymer- und Kolloidforschung Profilfelder > Emerging Fields > Lebensmittel- und Gesundheitswissenschaften Profilfelder Profilfelder > Advanced Fields Profilfelder > Emerging Fields |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 600 Technik, Medizin, angewandte Wissenschaften 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften |
| Eingestellt am: | 22 Sep 2015 09:04 |
| Letzte Änderung: | 26 Aug 2022 05:56 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/19489 |