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The yeast Sup35NM domain propagates as a prion in mammalian cells

Title data

Krammer, Carmen ; Kryndushkin, Dmitry ; Suhre, Michael H. ; Kremmer, Elisabeth ; Hofmann, Andreas ; Pfeifer, Alexander ; Scheibel, Thomas ; Wickner, Reed B. ; Schätzl, Hermann M. ; Vorberg, Ina M.:
The yeast Sup35NM domain propagates as a prion in mammalian cells.
In: Proceedings of the National Academy of Sciences of the United States of America. Vol. 106 (January 2009) Issue 2 . - pp. 462-467.
ISSN 1091-6490
DOI: https://doi.org/10.1073/pnas.0811571106

Abstract in another language

Prions are infectious, self-propagating amyloid-like protein aggregates of mammals and fungi. We have studied aggregation propensities of a yeast prion domain in cell culture to gain insights into general mechanisms of prion replication in mammalian cells. Here, we report the artificial transmission of a yeast prion across a phylogenetic kingdom. HA epitope-tagged yeast Sup35p prion domain NM was stably expressed in murine neuroblastoma cells. Although cytosolically expressed NM-HA remained soluble, addition of fibrils of bacterially produced Sup35NM to the medium efficiently induced appearance of phenotypically and biochemically distinct NM-HA aggregates that were inherited by daughter cells. Importantly, NM-HA aggregates also were infectious to recipient mammalian cells expressing soluble NM-HA and, to a lesser extent, to yeast. The fact that the yeast Sup35NM domain can propagate as a prion in neuroblastoma cells strongly argues that cellular mechanisms support prion-like inheritance in the mammalian cytosol.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: PrP; Sup35
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Emerging Fields > Food and Health Sciences
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Emerging Fields
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 22 Sep 2015 08:24
Last Modified: 26 Nov 2015 10:51
URI: https://eref.uni-bayreuth.de/id/eprint/19493