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Molecular design of performance proteins with repetitive sequences: recombinant flagelliform spider silk as basis for biomaterials.

Titelangaben

Vendrely, Charlotte ; Ackerschott, Christian B. ; Römer, Lin ; Scheibel, Thomas:
Molecular design of performance proteins with repetitive sequences: recombinant flagelliform spider silk as basis for biomaterials.
In: Gazit, Ehud ; Nussinov, Ruth (Hrsg.): Nanostructure design : methods and protocols. - Totowa, NJ : Humana Press , 2008 . - S. 3-14 . - (Methods in Molecular Biology ; 474 )
ISBN 978-1-934115-35-0
DOI: https://doi.org/10.1007/978-1-59745-480-3_1

Abstract

Most performance proteins responsible for the mechanical stability of cells and organisms reveal highly repetitive sequences. Mimicking such performance proteins is of high interest for the design of nanostructured biomaterials. In this article, flagelliform silk is exemplary introduced to describe a general principle for designing genes of repetitive performance proteins for recombinant expression in Escherichia coli . In the first step, repeating amino acid sequence motifs are reversely transcripted into DNA cassettes, which can in a second step be seamlessly ligated, yielding a designed gene. Recombinant expression thereof leads to proteins mimicking the natural ones. The recombinant proteins can be assembled into nanostructured materials in a controlled manner, allowing their use in several applications.

Weitere Angaben

Publikationsform: Aufsatz in einem Buch
Begutachteter Beitrag: Ja
Keywords: Biomaterials; recombinant production; repetitive sequence; spider silk proteins
Institutionen der Universität: Fakultäten
Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien
Profilfelder > Advanced Fields > Neue Materialien
Profilfelder > Advanced Fields > Molekulare Biowissenschaften
Profilfelder > Advanced Fields > Polymer- und Kolloidforschung
Profilfelder > Emerging Fields > Lebensmittel- und Gesundheitswissenschaften
Profilfelder
Profilfelder > Advanced Fields
Profilfelder > Emerging Fields
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 600 Technik, Medizin, angewandte Wissenschaften
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Eingestellt am: 23 Sep 2015 12:28
Letzte Änderung: 23 Apr 2024 10:55
URI: https://eref.uni-bayreuth.de/id/eprint/19496