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Vendrely, Charlotte ; Ackerschott, Christian B. ; Römer, Lin ; Scheibel, Thomas:
Molecular design of performance proteins with repetitive sequences: recombinant flagelliform spider silk as basis for biomaterials.
In: Methods in Molecular Biology.
Bd. 474
(2008)
.
- S. 3-14.
ISSN 1940-6029
DOI: https://doi.org/10.1007/978-1-59745-480-3_1
Abstract
Most performance proteins responsible for the mechanical stability of cells and organisms reveal highly repetitive sequences. Mimicking such performance proteins is of high interest for the design of nanostructured biomaterials. In this article, flagelliform silk is exemplary introduced to describe a general principle for designing genes of repetitive performance proteins for recombinant expression in Escherichia coli . In the first step, repeating amino acid sequence motifs are reversely transcripted into DNA cassettes, which can in a second step be seamlessly ligated, yielding a designed gene. Recombinant expression thereof leads to proteins mimicking the natural ones. The recombinant proteins can be assembled into nanostructured materials in a controlled manner, allowing their use in several applications.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Keywords: | Biomaterials; recombinant production; repetitive sequence; spider silk proteins |
| Institutionen der Universität: | Fakultäten Fakultäten > Fakultät für Ingenieurwissenschaften Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Biomaterialien Profilfelder > Advanced Fields > Neue Materialien Profilfelder > Advanced Fields > Molekulare Biowissenschaften Profilfelder > Advanced Fields > Polymer- und Kolloidforschung Profilfelder > Emerging Fields > Lebensmittel- und Gesundheitswissenschaften Profilfelder Profilfelder > Advanced Fields Profilfelder > Emerging Fields |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 600 Technik, Medizin, angewandte Wissenschaften 600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften |
| Eingestellt am: | 23 Sep 2015 12:28 |
| Letzte Änderung: | 21 Sep 2016 06:36 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/19496 |