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Molecular design of performance proteins with repetitive sequences: recombinant flagelliform spider silk as basis for biomaterials.

Title data

Vendrely, Charlotte ; Ackerschott, Christian B. ; Römer, Lin ; Scheibel, Thomas:
Molecular design of performance proteins with repetitive sequences: recombinant flagelliform spider silk as basis for biomaterials.
In: Methods in Molecular Biology. Vol. 474 (2008) . - pp. 3-14.
ISSN 1940-6029
DOI: https://doi.org/10.1007/978-1-59745-480-3_1

Abstract in another language

Most performance proteins responsible for the mechanical stability of cells and organisms reveal highly repetitive sequences. Mimicking such performance proteins is of high interest for the design of nanostructured biomaterials. In this article, flagelliform silk is exemplary introduced to describe a general principle for designing genes of repetitive performance proteins for recombinant expression in Escherichia coli . In the first step, repeating amino acid sequence motifs are reversely transcripted into DNA cassettes, which can in a second step be seamlessly ligated, yielding a designed gene. Recombinant expression thereof leads to proteins mimicking the natural ones. The recombinant proteins can be assembled into nanostructured materials in a controlled manner, allowing their use in several applications.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: Biomaterials; recombinant production; repetitive sequence; spider silk proteins
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Emerging Fields > Food and Health Sciences
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Emerging Fields
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 23 Sep 2015 12:28
Last Modified: 21 Sep 2016 06:36
URI: https://eref.uni-bayreuth.de/id/eprint/19496