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Assembly mechanism of recombinant spider silk proteins

Title data

Rammensee, Sebastian ; Slotta, Ute ; Scheibel, Thomas ; Bausch, Andreas R.:
Assembly mechanism of recombinant spider silk proteins.
In: Proceedings of the National Academy of Sciences of the United States of America. Vol. 105 (March 2008) Issue 18 . - pp. 6590-6595.
ISSN 1091-6490
DOI: https://doi.org/10.1073/pnas.0709246105

Abstract in another language

Spider silk threads are formed by the irreversible aggregation of silk proteins in a spinning duct with dimensions of only a few micrometers. Here, we present a microfluidic device in which engineered and recombinantly produced spider dragline silk proteins eADF3 (engineered Araneus diadematus fibroin) and eADF4 are assembled into fibers. Our approach allows the direct observation and identification of the essential parameters of dragline silk assembly. Changes in ionic conditions and pH result in aggregation of the two proteins. Assembly of eADF3 fibers was induced only in the presence of an elongational flow component. Strikingly, eADF4 formed fibers only in combination with eADF3. On the basis of these results, we propose a model for dragline silk aggregation and early steps of fiber assembly in the microscopic regime.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: colloids; microfluidics; Protein; materials; rheology
Institutions of the University: Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Profile Fields > Advanced Fields > Advanced Materials
Profile Fields > Advanced Fields > Molecular Biosciences
Profile Fields > Advanced Fields > Polymer and Colloid Science
Profile Fields > Emerging Fields > Food and Health Sciences
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Emerging Fields
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 24 Sep 2015 06:56
Last Modified: 20 May 2016 06:29
URI: https://eref.uni-bayreuth.de/id/eprint/19513