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Influence of nitrate on oxalate- and glyoxylate-dependent growth and acetogenesis by Moorella thermoacetica

Title data

Seifritz, Corinna ; Fröstl, Jürgen M. ; Drake, Harold L. ; Daniel, Steven L.:
Influence of nitrate on oxalate- and glyoxylate-dependent growth and acetogenesis by Moorella thermoacetica.
In: Archives of Microbiology. Vol. 178 (2002) Issue 6 . - pp. 457-464.
ISSN 1432-072X
DOI: https://doi.org/10.1007/s00203-002-0475-6

Abstract in another language

Oxalate and glyoxylate supported growth and acetate synthesis by Moorella thermoacetica in the presence of nitrate under basal (without yeast extract) culture conditions. In oxalate cultures, acetate formation occurred concomitant to growth, and nitrate was reduced in the stationary phase. Growth in the presence of [14C]bicarbonate or [14C]oxalate showed that CO2 reduction to acetate and biomass or oxalate oxidation to CO2 was not affected by nitrate. However, cells engaged in oxalate-dependent acetogenesis in the presence of nitrate lacked a membranous b-type cytochrome which was present in cells grown in the absence of nitrate. In glyoxylate cultures, growth was coupled to nitrate reduction, and acetate was formed in the stationary phase after nitrate was totally consumed. In the absence of nitrate, glyoxylate cells incorporated less CO2 into biomass than oxalate cells. CO2 conversion to biomass by glyoxylate cells decreased when cells were grown in the presence of nitrate. These results suggest that (i) oxalate cells prefer CO2 as an electron sink and bypass the nitrate block on the acetyl-CoA pathway at the level of reductant flow and (ii) glyoxylate cells prefer nitrate as an electron sink and bypass the nitrate block of the acetyl-CoA pathway by assimilating carbon via an unknown process that supplements or replaces the acetyl-CoA pathway. In this regard, enzymes of known pathways for the assimilation of two-carbon compounds were not detected in glyoxylate or oxalate cells.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER13511
Institutions of the University: Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 24 Sep 2015 09:38
Last Modified: 26 Jul 2016 08:24
URI: https://eref.uni-bayreuth.de/id/eprint/19613