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Thermicanus aegyptius gen. nov., sp. nov., isolated from oxic soil, a fermentative microaerophile that grows commensally with the thermophilic acetogen Moorella thermoacetica

Title data

Gößner, Anita S. ; Devereux, Richard ; Ohnemüller, Nadja ; Acker, Georg ; Stackebrandt, Erko ; Drake, Harold L.:
Thermicanus aegyptius gen. nov., sp. nov., isolated from oxic soil, a fermentative microaerophile that grows commensally with the thermophilic acetogen Moorella thermoacetica.
In: Applied and Environmental Microbiology. Vol. 65 (1999) Issue 11 . - pp. 5124-5133.
ISSN 1098-5336

Abstract in another language

A thermophilic, fermentative microaerophile (ET-5b) and a thermophilic acetogen (ET-5a) were co-isolated from oxic soil obtained from Egypt. The 16S rRNA gene sequence of ET-5a was 99.8% identical to that of the classic acetogen Moorella thermoacetica. Further analyses confirmed that ET-5a was a new strain of M. thermoacetica. For ET-5b, the nearest 16S rRNA gene sequence similarity value to known genera approximated 88%. ET-5b was a motile rod with a genomic G + C content of 50.3 mol%. Cells were weakly gram positive and lacked spores. Growth was optimal at 55-60°C and pH 6.5-7.0. ET-5b grew under both oxic and anoxic conditions, but growth was erratic under atmospheric concentrations of O2. Utilizable substrates included oligosaccharides and monosaccharides. Acetate, formate, and succinate were growth supportive only under oxic conditions. Saccharides yielded succinate, lactate, ethanol, acetate, formate, and H2 under anoxic conditions; fermentation products were also formed under oxic conditions. A new genus is proposed, the type strain being Thermicanus aegyptius ET-5bT gen. nov., sp. nov. (DSMZ 12793T) (T = type strain). M. thermoacetica ET-5a (DSMZ 12797) grew commensally with T. aegyptius ET-5b on oligosaccharides via the interspecies transfer of H2, formate, and lactate. In support of this interaction, uptake hydrogenase and formate dehydrogenase specific activities were fundamentally greater in M. thermoacetica ET-5a than in T. aegyptius ET-5b. These results demonstrate that (i) soils subject to high temperatures harbor uncharacterized thermophilic microaerophiles, (ii) the classic acetogen M. thermoacetica resides in such soils, and (iii) trophic links between such soil bacteria might contribute to their in situ activities.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER7404
Institutions of the University: Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 30 Sep 2015 05:56
Last Modified: 30 Sep 2015 05:56
URI: https://eref.uni-bayreuth.de/id/eprint/19925