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Carbonic anhydrase in Acetobacterium woodii and other acetogenic bacteria

Title data

Braus-Stromeyer, Susanna ; Schnappauf, Georg ; Braus, Gerhard H. ; Gößner, Anita S. ; Drake, Harold L.:
Carbonic anhydrase in Acetobacterium woodii and other acetogenic bacteria.
In: Journal of Bacteriology. Vol. 179 (1997) Issue 22 . - pp. 7197-7200.
ISSN 1098-5530

Abstract in another language

Acetobacterium woodii, Acetohalobium arabaticum, Clostridium formicoaceticum, and Sporomusa silvacetica contained carbonic anhydrase (CA). Minimal to no CA activity was detected in Moorella thermoautotrophica, Moorella thermoacetica subsp. 'pratumsolum', Sporomusa termitida, Thermoanaerobacter kivui. A. woodii contained the highest CA specific activity of the acetogens evaluated, with activities approximating 14 units mg (protein)-1 in cell extracts of either glucose- or H2-CO2-cultivated cells. CA of A. woodii was cytoplasmic and was purified approximately 300-fold to a specific activity of 5,236 units mg (protein)-1. Intracellular acetate concentrations inhibited CA activity of A. woodii by 50-85%, indicating that intracellular acetate may affect in situ CA activity.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER7183
Institutions of the University: Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 30 Sep 2015 05:56
Last Modified: 30 Sep 2015 05:56
URI: https://eref.uni-bayreuth.de/id/eprint/19980