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Chromatographic techniques in the downstream processing of proteins in biotechnology

Title data

Freitag, Ruth:
Chromatographic techniques in the downstream processing of proteins in biotechnology.
In: Pörtner, Ralf (ed.): Animal cell biotechnology : methods and protocols. - New York, NY : Humana Press , 2014 . - pp. 419-458 . - (Methods in Molecular Biology ; 1104 ) (Springer protocols)
ISBN 978-1-62703-732-7
DOI: https://doi.org/10.1007/978-1-62703-733-4_25

Official URL: Volltext

Abstract in another language

The purification of the product, the so-called downstream process (DSP), tends to be one of the most costly aspects of modern bioprocessing, especially in the case of proteins. In such cases, chromatography is still the major tool on all levels of the DSP from the first capture to the final polishing step. In this chapter, we will first outline the commonly used methods and their setup, in particular ion exchange chromatography (IEX), hydrophobic interaction chromatography (HIC), affinity chromatography (AC), and gel filtration (GPC, SEC), but also some less-known alternatives such as hydroxyapatite chromatography (HAC). Then the rational design of a downstream process, which usually comprises three orthogonal chromatographic steps, is discussed. Finally, process variants deviating from the usual batch-column/gradient elution approach will be presented, including expanded bed, displacement, and continuous chromatography, but also affinity precipitation. A most recent trend observable in the biotechnical DSP is the drive towards disposable elements (single-use technologies). Some options for this will be discussed as well.

Further data

Item Type: Article in a book
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 22 Feb 2016 15:19
Last Modified: 29 Feb 2016 08:23
URI: https://eref.uni-bayreuth.de/id/eprint/31019