Clickable, Biocompatible, and Fluorescent Hybrid Nanoparticles for Intracellular Delivery and Optical Imaging

Titelangaben

Müllner, Markus ; Schallon, Anja ; Walther, Andreas ; Freitag, Ruth ; Müller, Axel H. E.:
Clickable, Biocompatible, and Fluorescent Hybrid Nanoparticles for Intracellular Delivery and Optical Imaging.
In: Biomacromolecules. Bd. 11 (2010) Heft 2 . - S. 390-396.
ISSN 1526-4602
DOI: https://doi.org/10.1021/bm901099p

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Abstract

We report a general and facile approach for the fabrication of a new class of near monodisperse hybrid nanoparticles via RAFT polymerization and self-assembly in water. Furthermore, we combine a fluorescent inorganic silica core with a biocompatible polymer shell and a terminal unit susceptible to facile conjugations via click chemistry. A tailoring of the weight fractions of both components allows a tuning of the size of the formed aggregates. Fluorescent properties and the crosslinking into an organic-inorganic hybrid network are realized by copolymerizing a dye-functionalized monomer 1-pyrenebutyl acrylate and a trimethoxysilane-carrying one, (3-acryloxypropyl)trimethoxysilane. The potential of these stabilized and fluorescent nanoparticles as biocompatible carriers for intracellular delivery is demonstrated via in vitro experiments on lung cancer cells.