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Use of the avidin (imino)biotin system as a general approach to affinity precipitation

Title data

Freitag, Ruth ; Hilbrig, Frank:
Use of the avidin (imino)biotin system as a general approach to affinity precipitation.
In: McMahon, Robert J. (ed.): Avidin-Biotin interactions : methods and applications. - Totowa, NJ : Humana Press , 2008 . - pp. 35-50 . - (Methods in Molecular Biology ; 418 )
ISBN 978-1-588-29583-5

Abstract in another language

Biospecific interactions are used in many capturing and bioseparation steps. A typical situation is the coupling of a biospecific ligand to a chromatographic stationary phase for affinity chromatography. This approach has two possible drawbacks. The first is that a chromatographic column may be awkward to use in experimental setups; the second is related to the need to develop a dedicated coupling chemistry for any given affinity ligand. In affinity precipitation, the biospecific affinity ligand is instead linked to a stimuli-responsive molecule to yield a so-called affinity macroligand (AML). Upon stimulation, such molecules show abrupt yet reversible precipitation from aqueous solution. Capture by affinity precipitation just requires the addition of the stimuli-responsive AML to the raw target solution followed by selective precipitation of the formed affinity complex via the application of the stimulus. The need for the synthesis of a dedicated AML may be circumvented by the use of an avidin-activated stimuli-responsive precursor, to which any biotinylated affinity ligand can be securely linked via the well-known strong avidin-biotin interaction.

Further data

Item Type: Article in a book
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 24 Feb 2016 14:41
Last Modified: 17 May 2016 07:12
URI: https://eref.uni-bayreuth.de/id/eprint/31040