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A fast method for the quantification of methylamine in fermentation broths by gas chromatography

Title data

Jérôme, Valérie ; Hermann, Markus ; Hilbrig, Frank:
A fast method for the quantification of methylamine in fermentation broths by gas chromatography.
In: Journal of Chromatography B. Vol. 861 (January 2008) Issue 1 . - pp. 88-94.
ISSN 1873-376X
DOI: https://doi.org/10.1016/j.jchromb.2007.11.027

Official URL: Volltext

Abstract in another language

The objective of this study was to develop a method for the quantitative analysis of the methylamine concentration in fermentation broths of Hyphomicrobium zavarzinii ZV 580 cultures. For this purpose an established method for the quantification of free amino acids in such matrices was adapted and validated. The detection limit was 10 microM, the calibration curve showed good linearity (R2=0.9998) in the concentration range between 0.1 and 8 mM. The standard deviation of the injection-to-injection reproducibility (n=10) of the retention coefficient was <1%, that of the peak area<5%. In case of the sample-to-sample reproducibility (n=8), the standard deviation was <5% for the retention coefficient and <10% for the peak area. The validated method was successfully applied for monitoring a fed-batch bioprocess (starting volume: 8L, initial methylamine hydrochloride concentration: 10 mM) producing a dye-linked formaldehyde dehydrogenase in H. zavarzinii ZV 580.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Faculties
Result of work at the UBT: Yes
DDC Subjects: 600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 24 Feb 2016 15:07
Last Modified: 24 Feb 2016 15:07
URI: https://eref.uni-bayreuth.de/id/eprint/31041