Theory and practical understanding of the migration behavior of proteins and peptides in CE and related techniques

Titelangaben

Freitag, Ruth ; Hilbrig, Frank:
Theory and practical understanding of the migration behavior of proteins and peptides in CE and related techniques.
In: Electrophoresis. Bd. 28 (2007) Heft 13 . - S. 2125-2144.
ISSN 1522-2683
DOI: https://doi.org/10.1002/elps.200600792

Volltext

Link zum Volltext (externe URL):

Abstract

CEC is defined as an analytical method, where the analytes are separated on a chromatographic column in the presence of an applied voltage. The separation of charged analytes in CEC is complex, since chromatographic interaction, electroosmosis and electrophoresis contribute to the experimentally observed behavior. The putative contribution of effects such as surface electrodiffusion has been suggested. A sound theoretical treatment incorporating all effects is currently not available. The question of whether the different effects contribute in an independent or an interdependent manner is still under discussion. In this contribution, the state-of-the-art in the theoretical description of the individual contributions as well as models for the retention behavior and in particular possible dimensionless 'retention factors' is discussed, together with the experimental database for the separation of charged analytes, in particular proteins and peptides, by CEC and related techniques.