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Pumping of mammalian cells with a nozzle-diffuser micropump

Title data

Yamahata, Christophe ; Vandevyver, Caroline ; Lacharme, Frederic ; Izewska, Paulina ; Vogel, Horst ; Freitag, Ruth ; Gijs, Martin A. M.:
Pumping of mammalian cells with a nozzle-diffuser micropump.
In: Lab on a Chip. Vol. 5 (2005) Issue 10 . - pp. 1083-1088.
ISSN 1473-0189
DOI: https://doi.org/10.1039/B504468E

Official URL: Volltext

Abstract in another language

We discuss the successful transport of jurkat cells and 5D10 hybridoma cells using a reciprocating micropump with nozzle-diffuser elements. The effect of the pumping action on cell viability and proliferation, as well as on the damaging of cellular membranes is quantified using four types of well-established biological tests: a trypan blue solution, the tetrazolium salt WST-1 reagent, the LDH cytotoxicity assay and the calcium imaging ATP test. The high viability levels obtained after pumping, even for the most sensitive cells (5D10), indicate that a micropump with nozzle-diffuser elements can be very appropriate for handling living cells in cell-on-a-chip applications.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Result of work at the UBT: No
DDC Subjects: 500 Science
500 Science > 500 Natural sciences
600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 25 Feb 2016 14:08
Last Modified: 25 Feb 2016 14:08
URI: https://eref.uni-bayreuth.de/id/eprint/31052