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Light-responsive bioconjugates as novel tools for specific capture of biologicals by photoaffinity precipitation

Title data

Desponds, Arnaud ; Freitag, Ruth:
Light-responsive bioconjugates as novel tools for specific capture of biologicals by photoaffinity precipitation.
In: Biotechnology and Bioengineering. Vol. 91 (September 2005) Issue 5 . - pp. 583-591.
ISSN 1097-0290
DOI: https://doi.org/10.1002/bit.20479

Official URL: Volltext

Abstract in another language

Light-responsive bioconjugates are synthesized by a two-step protocol calling first for cotelomerization (chain-transfer polymerization) of N-isopropylacrylamide and N-acryloxysuccinimide. The desired bioligand (biotin) is used in modified form as chain-transfer agent in this step. As a consequence, 100 of the produced bioconjugates carry this group. In a second step, the cotelomers (bioconjugates) are rendered photoresponsive by linking a chromophore ((3-aminopropyloxy)azobenzene) group to the N-acryloxysuccinimide side chains. The resulting structures show a critical solution temperature in pure water of 16°C when the azo groups in the side chains are predominately in the (stable) trans state. Irradiation with UV light (330 nm) switches the azo group into the more hydrophilic cis state, and the critical solution temperature rises to 18°C. Irradiation with visible light (> 440 nm) switches the group back to the trans state. Adjusting the temperature to an intermediate level, the bioconjugates are used to demonstrate the concept of photo affinity precipitation, i.e., the specific capture and recovery by light-induced precipitation of a target molecule (avidin) from a serum-containing cell-culture supernatant. The avidin was obtained in highly purified form; no nonspecific copurification of protein impurities was observable

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: affinity precipitation; avidin; biotin, bioseparation, photoresponsive polymers; stimuli-responsive materials
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Faculties
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Result of work at the UBT: No
DDC Subjects: 500 Science
500 Science > 500 Natural sciences
600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 620 Engineering
Date Deposited: 25 Feb 2016 14:19
Last Modified: 25 Feb 2016 14:19
URI: https://eref.uni-bayreuth.de/id/eprint/31054