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Comparison of a three-peptide separation by capillary electrochromatography, voltage-assisted liquid chromatography and nano-high-performance liquid chromatography

Titelangaben

Szucs, Veronika ; Freitag, Ruth:
Comparison of a three-peptide separation by capillary electrochromatography, voltage-assisted liquid chromatography and nano-high-performance liquid chromatography.
In: Journal of Chromatography A. Bd. 1044 (Juli 2004) Heft 1/2 . - 201 - 210.
ISSN 1873-3778
DOI: https://doi.org/10.1016/j.chroma.2004.05.103

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Link zum Volltext (externe URL): Volltext

Abstract

A mixture of three peptides was separated by capillary electrochromatography (CEC), nano-HPLC and voltage-assisted LC. In the latter case the charged analytes migrate through a neutral stationary phase driven by electrophoresis while their interaction with the stationary phase provides the basis for a chromatographic separation. The stationary phases used were poly(glycidyl methacrylate-co-ethylene dimethacrylate)-based monoliths that could be used directly as neutral “C1”-type columns for voltage-assisted LC and nano-HPLC, while their application in CEC became possible after derivatization of the epoxy groups with ionogenic N-ethylbutylamine functions. The separation of the peptide mixture was possible in all three modes. Highest plate numbers and resolutions were obtained under voltage-assisted conditions. The elution order showed dependencies on the charge density but also on the hydrophobicity of the peptides and was different in the three investigated chromatographic modes. The effect of changes in the ionic strength and the organic solvent content of the mobile phase on the resolution and the migration behavior of the peptides was investigated and showed the expected behavior. Voltage-assisted LC is suggested as an alternative to {CEC} for the separation of charged analytes by electrochromatography.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: Peptides
Institutionen der Universität: Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik > Lehrstuhl Bioprozesstechnik - Univ.-Prof. Dr. Ruth Freitag
Fakultäten
Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik
Titel an der UBT entstanden: Nein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik
600 Technik, Medizin, angewandte Wissenschaften
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Eingestellt am: 25 Feb 2016 14:36
Letzte Änderung: 25 Feb 2016 14:36
URI: https://eref.uni-bayreuth.de/id/eprint/31058