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Continuous Separation of Multicomponent Protein Mixtures by Annular Displacement Chromatography

Titelangaben

Giovannini, Roberto ; Freitag, Ruth:
Continuous Separation of Multicomponent Protein Mixtures by Annular Displacement Chromatography.
In: Biotechnology Progress. Bd. 18 (2002) Heft 6 . - S. 1324-1331.
ISSN 1520-6033
DOI: https://doi.org/10.1021/bp010154y

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Link zum Volltext (externe URL): Volltext

Abstract

Displacement chromatography is a predominantly nonlinear mode of chromatography, which has certain advantages over the elution mode for preparative bioseparations. Whereas continuous production (and separation) processes have their theoretical benefits in this context, protein displacement chromatography has up to now only been performed in the batch mode. In this contribution, we demonstrate that the principle of continuous annular chromatography can be adapted to displacement chromatography. Separations of up to three standard proteins (two whey proteins, soybean trypsin inhibitor) were developed and optimized using a small (4 × 250 mm) batch column. These separations were subsequently transferred directly to the continuous system (500-mL column). Separations of similar quality in terms of final product purity and recovery yield were obtained using the continuous system.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Institutionen der Universität: Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik > Lehrstuhl Bioprozesstechnik - Univ.-Prof. Dr. Ruth Freitag
Fakultäten
Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik
Titel an der UBT entstanden: Nein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik
600 Technik, Medizin, angewandte Wissenschaften
600 Technik, Medizin, angewandte Wissenschaften > 620 Ingenieurwissenschaften
Eingestellt am: 25 Feb 2016 15:14
Letzte Änderung: 25 Feb 2016 15:14
URI: https://eref.uni-bayreuth.de/id/eprint/31067