Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

A synthetic leucine zipper-based dimerization system for combining multiple promoter specificities

Title data

Jérôme, Valérie ; Müller, Rolf:
A synthetic leucine zipper-based dimerization system for combining multiple promoter specificities.
In: Gene Therapy. Vol. 8 (May 2001) Issue 9 . - pp. 725-729.
ISSN 1476-5462

Official URL: Volltext

Abstract in another language

One of the biggest challenges facing gene therapy is the development of vectors that direct the activity of therapeutic genes specifically to the sites of disease. To achieve this goal, the restriction of transgene transcription via synthetic promoters that are endowed with multiple specificities represents a particularly promising strategy. Towards this end, we have developed a generally applicable strategy (DCTF system) where a synthetic promoter is driven by an artificial heterodimeric transcription factor whose DNA-binding and transactivating subunits are expressed from two promoters with different selectivity. A crucial determinant of the DCTF system is the heterodimerization interface that should provide for a high affinity interaction without interference by endogenous proteins. Here, we describe such a dimerization system based on engineered Fos and Jun leucine zippers. We show the usefulness of this system for the combination of cell type-specific and cell cycle-regulated transcription and demonstrate its functionality in an in vivo setting.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Faculties
Result of work at the UBT: No
DDC Subjects: 500 Science
500 Science > 500 Natural sciences
600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 610 Medicine and health
Date Deposited: 26 Feb 2016 10:53
Last Modified: 26 Feb 2016 10:53
URI: https://eref.uni-bayreuth.de/id/eprint/31080