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On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme


Kuhn, Claus-D. ; Wilusz, Jeremy E. ; Zheng, Yuxuan ; Beal, Peter A. ; Joshua-Tor, Leemor:
On-enzyme refolding permits small RNA and tRNA surveillance by the CCA-adding enzyme.
In: Cell. Bd. 160 (2015) Heft 4 . - S. 644-658.
ISSN 0092-8674
DOI: https://doi.org/10.1016/j.cell.2015.01.005


Link zum Volltext (externe URL): Volltext


Transcription in eukaryotes produces a number of long noncoding RNAs (lncRNAs). Two of these, MALAT1 and Menbeta, generate a tRNA-like small RNA in addition to the mature lncRNA. The stability of these tRNA-like small RNAs and bona fide tRNAs is monitored by the CCA-adding enzyme. Whereas CCA is added to stable tRNAs and tRNA-like transcripts, a second CCA repeat is added to certain unstable transcripts to initiate their degradation. Here, we characterize how these two scenarios are distinguished. Following the first CCA addition cycle, nucleotide binding to the active site triggers a clockwise screw motion, producing torque on the RNA. This ejects stable RNAs, whereas unstable RNAs are refolded while bound to the enzyme and subjected to a second CCA catalytic cycle. Intriguingly, with the CCA-adding enzyme acting as a molecular vise, the RNAs proofread themselves through differential responses to its interrogation between stable and unstable substrates.

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Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: Archaeoglobus fulgidus/*enzymology/metabolism; Base Sequence; Catalytic Domain; Humans; Mitochondria/*enzymology/metabolism; Models, Molecular; Nucleic Acid Conformation; RNA Nucleotidyltransferases/chemistry/genetics/*metabolism; RNA Stability; RNA, Small Untranslated/metabolism; RNA, Transfer/*chemistry/*metabolism
Institutionen der Universität: Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie
Profilfelder > Advanced Fields > Molekulare Biowissenschaften
Forschungseinrichtungen > Forschungszentren > Bayreuther Zentrum für Molekulare Biowissenschaften - BZMB
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften
Profilfelder > Advanced Fields
Forschungseinrichtungen > Forschungszentren
Titel an der UBT entstanden: Nein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
Eingestellt am: 10 Jan 2017 10:49
Letzte Änderung: 10 Jan 2017 10:49
URI: https://eref.uni-bayreuth.de/id/eprint/35636