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Compaction and transmembrane delivery of pDNA : differences between l-PEI and two types of amphiphilic block copolymers

Titelangaben

Raup, Alexander ; Wang, Hui ; Synatschke, Christopher V. ; Jérôme, Valérie ; Agarwal, Seema ; Pergushov, Dmitry V. ; Müller, Axel H. E. ; Freitag, Ruth:
Compaction and transmembrane delivery of pDNA : differences between l-PEI and two types of amphiphilic block copolymers.
In: Biomacromolecules. Bd. 18 (2017) Heft 3 . - S. 808-818.
ISSN 1526-4602
DOI: 10.1021/acs.biomac.6b01678

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Projektfinanzierung: Andere

Abstract

Polycations are popular agents for non-viral delivery of DNA to mammalian cells. Adding hydrophobic, biodegradable, or cell penetrating functions could help to improve their performance, which at present is below that of viral agents. A crucial first step in gene delivery is the complexation of the DNA. The characteristics of these ‘polyplexes’ presumably influence or even determine the subsequent steps of membrane passage, intracellular travelling / DNA release, and nuclear up-take. Herein, polyplexes formed with linear poly(ethylenimine) (l-PEI) are compared to complexes generated with functionalized diblock copolymers. While l-PEI interacts only electrostatically with the DNA, interaction in case of the diblock polymers may be mixed-mode. In certain cases transfection efficiency improved when the polyplexes were formed in hypertonic solution. Moreover, whereas conventional PEI-based polyplexes enter the cells via endocytosis, at least one of the diblock agents seemed to promote entry via transient destabilization of the plasma membrane.

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Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: gene delivery; cellular uptake; PDMAEMA; l-PEI; PHMG-PCL block copolymers
Institutionen der Universität: Fakultäten > Fakultät für Ingenieurwissenschaften
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik
Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik > Lehrstuhl Bioprozesstechnik - Univ.-Prof. Dr. Ruth Freitag
Fakultäten
Titel an der UBT entstanden: Ja
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 500 Naturwissenschaften
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
Eingestellt am: 24 Feb 2017 09:42
Letzte Änderung: 26 Apr 2017 09:52
URI: https://eref.uni-bayreuth.de/id/eprint/36185