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Compaction and transmembrane delivery of pDNA : differences between l-PEI and two types of amphiphilic block copolymers

Title data

Raup, Alexander ; Wang, Hui ; Synatschke, Christopher V. ; Jérôme, Valérie ; Agarwal, Seema ; Pergushov, Dmitry V. ; Müller, Axel H. E. ; Freitag, Ruth:
Compaction and transmembrane delivery of pDNA : differences between l-PEI and two types of amphiphilic block copolymers.
In: Biomacromolecules. Vol. 18 (2017) Issue 3 . - pp. 808-818.
ISSN 1526-4602
DOI: https://doi.org/10.1021/acs.biomac.6b01678

Project information

Project financing: Andere

Abstract in another language

Polycations are popular agents for non-viral delivery of DNA to mammalian cells. Adding hydrophobic, biodegradable, or cell penetrating functions could help to improve their performance, which at present is below that of viral agents. A crucial first step in gene delivery is the complexation of the DNA. The characteristics of these ‘polyplexes’ presumably influence or even determine the subsequent steps of membrane passage, intracellular travelling / DNA release, and nuclear up-take. Herein, polyplexes formed with linear poly(ethylenimine) (l-PEI) are compared to complexes generated with functionalized diblock copolymers. While l-PEI interacts only electrostatically with the DNA, interaction in case of the diblock polymers may be mixed-mode. In certain cases transfection efficiency improved when the polyplexes were formed in hypertonic solution. Moreover, whereas conventional PEI-based polyplexes enter the cells via endocytosis, at least one of the diblock agents seemed to promote entry via transient destabilization of the plasma membrane.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: gene delivery; cellular uptake; PDMAEMA; l-PEI; PHMG-PCL block copolymers
Institutions of the University: Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Faculties
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 570 Life sciences, biology
600 Technology, medicine, applied sciences > 610 Medicine and health
Date Deposited: 24 Feb 2017 09:42
Last Modified: 26 Apr 2017 09:52
URI: https://eref.uni-bayreuth.de/id/eprint/36185