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Kaiser, Patrick ; Werner, Melanie ; Jérôme, Valérie ; Freitag, Ruth:
Scale‐up of the ex vivo expansion of encapsulated primary human T lymphocytes.
In: Biotechnology and Bioengineering.
Bd. 115
(2018)
Heft 10
.
- S. 2632-2642.
ISSN 1097-0290
DOI: https://doi.org/10.1002/bit.26786
Abstract
A number of evolving medical therapies call for the controlled expansion of primary human T lymphocytes. After encapsulation in sodium cellulose sulfate–poly(diallyldimethyl) ammonium chloride polyelectrolyte capsules, T lymphocytes can be expanded without persisting activation. Here, the challenge of scaling up this process is addressed. Encapsulated T lymphocytes were cultured in spinner flasks as well as in several types of the bioreactor, including fixed and fluidized beds, a waved cell bag, and a standard stirred tank reactor (STR; 1‐L scale). Two proprietary T lymphocyte culture media as well as a standard RPMI‐based medium were used. As before, encapsulation coincided with the presence of only a low fraction of activated T lymphocytes (peripheral blood T cells) in the total population. Unexpectedly, growth rates were lower in well‐mixed reactors than those in cultivations under static conditions, that is, in T‐flasks. Switching the STR to low oxygen conditions (40% air saturation) improved the growth rates to the level of the static cultures and thus forms the potential basis for efficient scale‐up of T lymphocyte expansion. Results are presented on the ex vivo expansion of primary human T cells in T‐flasks and spinner flasks, as well as in several types of the bioreactor, including packed and fluidized beds, waved cell bags, and a standard stirred tank reactor.
Weitere Angaben
| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Zusätzliche Informationen: | Online version of record before inclusion in an issue |
| Keywords: | biomimetic microenvironment; bioreactor; encapsulation; human T lymphocytes; polyelectrolyte |
| Institutionen der Universität: | Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik > Lehrstuhl Bioprozesstechnik - Univ.-Prof. Dr. Ruth Freitag Fakultäten Fakultäten > Fakultät für Ingenieurwissenschaften Fakultäten > Fakultät für Ingenieurwissenschaften > Lehrstuhl Bioprozesstechnik |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie 600 Technik, Medizin, angewandte Wissenschaften 600 Technik, Medizin, angewandte Wissenschaften > 600 Technik 600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit |
| Eingestellt am: | 25 Jul 2018 08:30 |
| Letzte Änderung: | 27 Jan 2022 13:53 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/45236 |