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Grimmler, Christina ; Kuhfuß, Tamara ; Heiden, Matthias ; Schmidt, Heinar:
Non-invasive assessment of the bioburden of minced pork using a hand-held fluorescence device.
In: Technisches Messen.
Bd. 85
(2018)
Heft 3
.
- S. 177-183.
ISSN 2196-7113
DOI: https://doi.org/10.1515/teme-2017-0092
Abstract
To speed-up monitoring of the hygienic status of meat, fast and non-invasive measuring techniques are required. The feasibility of a hand-held fluorescence device (freshdetect) was investigated to quantify the bioburden of minced pork meat. In total, 144 minced pork samples from 18 batches were stored at 2°C for up to 8 days. Fluorescence spectra, L*a*b* colour and total viable mesophilic plate counts (TVC) were measured in parallel during this period. Correlation of the fluorescence spectra with log(TVC/g) using partial least squares regression yielded cross-validated predictions with R²cv = 0.72 and a prediction error of RMSECV = 0.97 log(TVC/g). Cross-validated limits of detection and quantification were determined as 1.03 and 3.38 log(TVC/g), respectively. This would allow for a quantification well below the alarm threshold of 5.7-6.7 log(TVC/g). The spectra correlated also well with storage time (R²cv = 0.81), but less strong with L*a*b* colour.