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Size Exclusion High Performance Liquid Chromatography : Re-Discovery of a Rapid and Versatile Method for Clean-Up and Fractionation in Chemical Ecology

Title data

Sperling, Sergej ; Kühbandner, Stephan ; Engel, Katharina C. ; Steiger, Sandra ; Stökl, Johannes ; Ruther, Joachim:
Size Exclusion High Performance Liquid Chromatography : Re-Discovery of a Rapid and Versatile Method for Clean-Up and Fractionation in Chemical Ecology.
In: Journal of Chemical Ecology. Vol. 41 (2015) Issue 6 . - pp. 574-583.
ISSN 1573-1561
DOI: https://doi.org/10.1007/s10886-015-0584-8

Abstract in another language

Solvent extraction of bioactive molecules from glands, tissues, or whole organisms is a common first step in chemoecological studies. Co-extraction of a surplus of high boiling materials such as triacylglycerides (TAGs) and other lipids with higher molecular weight might hamper the identification of volatile or medium-volatile semiochemicals by high resolution chromatographic and spectroscopic techniques. Therefore, effective clean-up procedures are needed to separate potential semiochemicals from the accompanying materials. Size exclusion high performance liquid chromatography (SE-HPLC), a technique often disregarded by chemoecologists, has proved to be a rapid and efficient clean-up method for complex crude extracts. We demonstrated that TAGs can be baseline separated from typical semiochemicals within less than 10 min on a porous gel stationary phase based on highly cross-linked polystyrene/divinylbenzene. We applied the method as a rapid one-step clean-up procedure for the analysis of juvenile hormone III in insect hemolymph by gas chromatography–mass spectrometry. We furthermore introduced some recent application examples on insect pheromones to demonstrate that SE-HPLC is not only an effective method for the purification of crude extracts, but can as well be used as a first fractionation step for the bioassay-guided identification of behavior modifying natural products. SE-HPLC can be well operated with low-boiling solvents such as dichloromethane, and results in fraction volumes of typically less than one ml, which decreases the danger of losing volatile analytes during subsequent concentration steps.

Further data

Item Type: Article in a journal
Refereed: Yes
Additional notes: BAYCEER147418
Keywords: Cuticular hydrocarbons; Juvenile hormone; Pheromones; Sample preparation; Triacylglycerides
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Animal Ecology II - Evolutionary Animal Ecology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Animal Ecology II - Evolutionary Animal Ecology > Chair Animal Ecology II - Evolutionary Animal Ecology - Univ.-Prof. Dr. Sandra Steiger
Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Result of work at the UBT: No
DDC Subjects: 500 Science
Date Deposited: 11 Apr 2019 13:57
Last Modified: 11 Apr 2019 13:57
URI: https://eref.uni-bayreuth.de/id/eprint/48348