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Redesign of LAOBP to bind novel l-amino acid ligands

Title data

Banda-Vázquez, Jesús ; Shanmugaratnam, Sooruban ; Rodríguez-Sotres, Rogelio ; Torres-Larios, Alfredo ; Höcker, Birte ; Sosa-Peinado, Alejandro:
Redesign of LAOBP to bind novel l-amino acid ligands.
In: Protein Science. Vol. 27 (May 2018) Issue 5 . - pp. 957-968.
ISSN 1469-896X
DOI: https://doi.org/10.1002/pro.3403

Project information

Project financing: Deutsche Forschungsgemeinschaft
DFG:HO4022/2-3

Abstract in another language

Computational protein design is still a challenge for advancing structure-function relationships. While recent advances in this field are promising, more information for genuine predictions is needed. Here, we discuss different approaches applied to install novel glutamine (Gln) binding into the Lysine/Arginine/Ornithine binding protein (LAOBP) from Salmonella typhimurium. We studied the ligand binding behavior of two mutants: a binding pocket grafting design based on a structural superposition of LAOBP to the Gln binding protein QBP from Escherichia coli and a design based on statistical coupled positions. The latter showed the ability to bind Gln even though the protein was not very stable. Comparison of both approaches highlighted a nonconservative shared point mutation between LAOBP_graft and LAOBP_sca. This context dependent L117K mutation in LAOBP turned out to be sufficient for introducing Gln binding, as confirmed by different experimental techniques. Moreover, the crystal structure of LAOBP_L117K in complex with its ligand is reported.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: binding pocket grafting; ligand specificity; protein design; statistical coupling analysis
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Birte Höcker
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 13 May 2019 09:05
Last Modified: 13 May 2019 09:05
URI: https://eref.uni-bayreuth.de/id/eprint/48919