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SEMPRE: Spectral Editing Mediated by Paramagnetic Relaxation Enhancement

Title data

Kellner, Ruth ; Mangels, Christian ; Schweimer, Kristian ; Prasch, Stefan J. ; Weiglmeier, Philipp Richard ; Rösch, Paul ; Schwarzinger, Stephan:
SEMPRE: Spectral Editing Mediated by Paramagnetic Relaxation Enhancement.
In: Journal of the American Chemical Society. Vol. 131 (2009) Issue 50 . - pp. 18016-18017.
ISSN 1520-5126
DOI: https://doi.org/10.1021/ja905412z

Official URL: Volltext

Abstract in another language

Paramagnetic relaxation enhancement (PRE) has become a useful and widely applied tool in biomolecular NMR spectroscopy. In particular investigations of large complexes or transient contacts benefit from PRE effects. Frequently such studies involve modification of the biomacromolecules under study. We here present a method for editing NMR spectra by utilizing a soluble gadolinium complex that broadens nuclear spins being at or close to the macromolecule−solvent interface. NOE signals in NOESY spectra are selectively attenuated if surface exposed nuclear spins are involved. HSQC-type spectra with paramagnetic agent contain only signals of the interior of the protein, while the corresponding difference spectra harbor signals allocated to surface spins. Thus, the number of signals can be reduced helping to minimize spectral overlap in large proteins. The method reveals additional information about the localization of spins being helpful for structure determination of large complexes.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers > Lehrstuhl Biopolymere - Apl. Prof. Dr. Birgitta Wöhrl
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 500 Natural sciences
500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 19 Dec 2014 08:19
Last Modified: 11 Jun 2019 09:07
URI: https://eref.uni-bayreuth.de/id/eprint/5129