Titlebar

Export bibliographic data
Literature by the same author
plus on the publication server
plus at Google Scholar

 

Genome analysis provides insights into microaerobic toluene-degradation pathway of Zoogloea oleivorans Bucᵀ

Title data

Táncsics, András ; Farkas, Milán ; Horváth, Balázs ; Maróti, Gergely ; Bradford, Lauren M. ; Lüders, Tillmann ; Kriszt, Balázs:
Genome analysis provides insights into microaerobic toluene-degradation pathway of Zoogloea oleivorans Bucᵀ.
In: Archives of Microbiology. (2019) .
ISSN 1432-072X
DOI: https://doi.org/10.1007/s00203-019-01743-8

Abstract in another language

Zoogloea oleivorans, capable of using toluene as a sole source of carbon and energy, was earlier found to be an active degrader under microaerobic conditions in aquifer samples. To uncover the genetic background of the ability of microaerobic toluene degradation in Z. oleivorans, the whole-genome sequence of the type strain BucT was revealed. Metatranscriptomic sequence reads, originated from a previous SIP study on microaerobic toluene degradation, were mapped on the genome. The genome (5.68 Mb) had a mean G + C content of 62.5%, 5005 protein coding gene sequences and 80 RNA genes. Annotation predicted that 66 genes were involved in the metabolism of aromatic compounds. Genome analysis revealed the presence of a cluster with genes coding for a multicomponent phenol-hydroxylase system and a complete catechol meta-cleavage pathway. Another cluster flanked by mobile-element protein coding genes coded a partial catechol meta-cleavage pathway including a subfamily I.2.C-type extradiol dioxygenase. Analysis of metatranscriptomic data of a microaerobic toluene-degrading enrichment, containing Z .  oleivorans as an active-toluene degrader revealed that a toluene dioxygenase-like enzyme was responsible for the ring-hydroxylation, while enzymes of the partial catechol meta-cleavage pathway coding cluster were responsible for further degradation of the aromatic ring under microaerobic conditions. This further advances our understanding of aromatic hydrocarbon degradation between fully oxic and strictly anoxic conditions.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Research Institutions
Research Institutions > Research Centres
Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology > Chair Ecological Microbiology - Univ.-Prof. Dr. Tillmann Lüders
Result of work at the UBT: Yes
DDC Subjects: 500 Science
Date Deposited: 20 Jan 2020 14:12
Last Modified: 20 Jan 2020 14:12
URI: https://eref.uni-bayreuth.de/id/eprint/53902