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Fast Mapping of Biomolecular Interfaces by Random Spin Labeling (RSL)

Title data

Hartl, Maximilian J. ; Burmann, Björn M. ; Prasch, Stefan J. ; Schwarzinger, Clemens ; Schweimer, Kristian ; Wöhrl, Birgitta M. ; Rösch, Paul ; Schwarzinger, Stephan:
Fast Mapping of Biomolecular Interfaces by Random Spin Labeling (RSL).
In: Journal of Biomolecular Structure & Dynamics. Vol. 29 (2012) Issue 4 . - pp. 793-798.
ISSN 0739-1102

Abstract in another language

Random spin labeling (RSL) is a method for rapid mapping of biomolecular interaction surfaces using an interaction partner with SL and an interaction partner enriched in 13C or 15N nuclei for paramagnetic relaxation enhanced NMR-based detection. The SL reaction is conducted in a manner resulting in a heterogeneous reaction product consisting of different populations of the protein carrying a varying number of spin labels at different positions. Preparation of the paramagnetic probe is complete within a few hours and hence much faster than site selective SL. RSL is applicable to tightly interacting systems but shows its particular strength when applied to systems involving weak or transient contacts.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers > Lehrstuhl Biopolymere - Apl. Prof. Dr. Birgitta Wöhrl
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 500 Natural sciences
500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 14 Jan 2015 07:50
Last Modified: 15 May 2019 08:32
URI: https://eref.uni-bayreuth.de/id/eprint/5489