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Transcriptional regulation of the Nε‐fructoselysine metabolism in Escherichia coli by global and substrate‐specific cues

Title data

Graf von Armansperg, Benedikt ; Koller, Franziska ; Gericke, Nicola ; Hellwig, Michael ; Jagtap, Pravin Kumar Ankush ; Heermann, Ralf ; Hennig, Janosch ; Henle, Thomas ; Lassak, Jürgen:
Transcriptional regulation of the Nε‐fructoselysine metabolism in Escherichia coli by global and substrate‐specific cues.
In: Molecular Microbiology. Vol. 115 (2021) Issue 2 . - pp. 175-190.
ISSN 1365-2958
DOI: https://doi.org/10.1111/mmi.14608

Abstract in another language

Thermally processed food is an important part of the human diet. Heat-treatment, however, promotes the formation of so-called Amadori rearrangement products, such as fructoselysine. The gut microbiota including Escherichia coli can utilize these compounds as a nutrient source. While the degradation route for fructoselysine is well described, regulation of the corresponding pathway genes frlABCD remained poorly understood. Here, we used bioinformatics combined with molecular and biochemical analyses and show that fructoselysine metabolism in E. coli is tightly controlled at the transcriptional level. The global regulator CRP (CAP) as well as the alternative sigma factor σ32 (RpoH) contribute to promoter activation at high cAMP-levels and inside warm-blooded hosts, respectively. In addition, we identified and characterized a transcriptional regulator FrlR, encoded adjacent to frlABCD, as fructoselysine-6-phosphate specific repressor. Our study provides profound evidence that the interplay of global and substrate-specific regulation is a perfect adaptation strategy to efficiently utilize unusual substrates within the human gut environment.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: 1-(ε-N-lysyl)-1-deoxy-D-fructose; Amadori rearrangement product; GntR transcriptional regulator; fructosyllysine; glycation
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry > Chair Biochemistry with an Emphasis on Biophysical Chemistry - Univ.-Prof. Dr. Janosch Hennig
Result of work at the UBT: No
DDC Subjects: 500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 06 Oct 2021 09:33
Last Modified: 06 Oct 2021 09:33
URI: https://eref.uni-bayreuth.de/id/eprint/67200