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A versatile assay platform for enzymatic poly(ethylene-terephthalate) degradation

Title data

Weigert, Sebastian ; Gagsteiger, Andreas ; Menzel, Teresa ; Höcker, Birte:
A versatile assay platform for enzymatic poly(ethylene-terephthalate) degradation.
In: Protein Engineering, Design & Selection. Vol. 34 (24 August 2021) . - 9 S..
ISSN 1741-0134
DOI: https://doi.org/10.1093/protein/gzab022

Official URL: Volltext

Project information

Project title:
Project's official titleProject's id
SFB 1357 MikroplastikSFB1357

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

Accumulation of plastic and subsequent microplastic is a major environmental challenge. With the discovery of potent polyethylene terephthalate (PET)-degrading enzymes, a new perspective arose for environmental decomposition as well as technical recycling. To explore the enormous diversity of potential PET-degrading enzymes in nature and also to conveniently employ techniques like protein engineering and directed evolution, a fast and reliable assay platform is needed. In this study we present our versatile solution applying a PET coating on standard lab consumables such as polymerase chain reaction tubes, 96- and 384-well microtiter plates, yielding an adjustable crystallinity of the PET. Combining the reaction vessels with either ultra-high performance liquid chromatography (UHPLC) or fluorometric readout and additional enzyme quantification offers a range of advantages. Thereby, the platform can easily be adapted to diverse needs from detailed analysis with high precision to high-throughput (HT) applications including crude lysate analysis.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Birte Höcker
Faculties > Faculty of Engineering Science
Faculties > Faculty of Engineering Science > Chair Polymer Materials
Research Institutions > Collaborative Research Centers, Research Unit > SFB 1357 - MIKROPLASTIK
Research Institutions
Research Institutions > Collaborative Research Centers, Research Unit
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 500 Natural sciences
500 Science > 540 Chemistry
500 Science > 570 Life sciences, biology
Date Deposited: 19 Oct 2021 08:16
Last Modified: 20 Oct 2021 06:12
URI: https://eref.uni-bayreuth.de/id/eprint/67372