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Tailor-Made Protein Corona Formation on Polystyrene Microparticles and its Effect on Epithelial Cell Uptake

Title data

Jasinski, Julia ; Wilde, Magdalena V. ; Völkl, Matthias ; Jérôme, Valérie ; Fröhlich, Thomas ; Freitag, Ruth ; Scheibel, Thomas:
Tailor-Made Protein Corona Formation on Polystyrene Microparticles and its Effect on Epithelial Cell Uptake.
In: ACS Applied Materials & Interfaces. Vol. 14 (4 October 2022) Issue 41 . - pp. 47277-47287.
ISSN 1944-8252
DOI: https://doi.org/10.1021/acsami.2c13987

Project information

Project financing: Deutsche Forschungsgemeinschaft

Abstract in another language

Microplastic particles are pollutants in the environment with a potential impact on ecology and human health. As soon as microplastic particles get in contact with complex (biological) environments, they will be covered by an eco- and/or protein corona. In this contribution, protein corona formation was conducted under defined laboratory conditions on polystyrene (PS) microparticles to investigate the influence on surface properties, protein corona evolution, particle–cell interactions, and uptake in two murine epithelial cells. To direct protein corona formation, PS particles were preincubated with five model proteins, namely, bovine serum albumin (BSA), myoglobin, β-lactoglobulin, lysozyme, and fibrinogen. Subsequently, the single-protein-coated particles were incubated in a cell culture medium containing a cocktail of serum proteins to analyze changes in the protein corona profile as well as in the binding kinetics of the model proteins. Therein, we could show that the precoating step has a critical impact on the final composition of the protein corona. Yet, since proteins building the primary corona were still detectable after additional incubations in a protein-containing medium, backtracking of the particle’s history is possible. Interestingly, whereas the precoating history significantly disturbs particle–cell interactions (PCIs), the cellular response (i.e., metabolic activity, MTT assay) stays unaffected. Of note, lysozyme precoating revealed one of the highest rates in PCI for both epithelial cell lines. Taken together, we could show that particle history has a significant impact on protein corona formation and subsequently on the interaction of particles with murine intestinal epithelial-like cells. However, as this study was limited to one cell type, further work is needed to assess if these observations can be generalized to other cell types.

Further data

Item Type: Article in a journal
Refereed: Yes
Keywords: microplastic; particle history; particle−cell interaction; particle ingestion; cytotoxicity; LC−MS/MS, QCM-D
Institutions of the University: Faculties > Faculty of Engineering Science > Chair Process Biotechnology
Faculties > Faculty of Engineering Science > Chair Process Biotechnology > Chair Process Biotechnology - Univ.-Prof. Dr. Ruth Freitag
Faculties > Faculty of Engineering Science > Chair Biomaterials
Faculties > Faculty of Engineering Science > Chair Biomaterials > Chair Biomaterials - Univ.-Prof. Dr. Thomas Scheibel
Research Institutions > Collaborative Research Centers, Research Unit > SFB 1357 - MIKROPLASTIK
Faculties
Faculties > Faculty of Engineering Science
Research Institutions
Research Institutions > Collaborative Research Centers, Research Unit
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
600 Technology, medicine, applied sciences
600 Technology, medicine, applied sciences > 600 Technology
Date Deposited: 10 Oct 2022 07:01
Last Modified: 14 Nov 2022 13:23
URI: https://eref.uni-bayreuth.de/id/eprint/72335