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CDC-48/p97 Coordinates CDT1 Degradation with GINS Chromatin Dissociation to Ensure Faithful DNA Replication

Title data

Franz, André ; Orth, Michael ; Pirson, Paul A. ; Sonneville, Remi ; Blow, J. Julian ; Gartner, Anton ; Stemmann, Olaf ; Hoppe, Thorsten:
CDC-48/p97 Coordinates CDT1 Degradation with GINS Chromatin Dissociation to Ensure Faithful DNA Replication.
In: Molecular Cell. Vol. 44 (7 October 2011) Issue 1 . - pp. 85-96.
ISSN 1097-4164
DOI: https://doi.org/10.1016/j.molcel.2011.08.028

Official URL: Volltext

Abstract in another language

Faithful transmission of genomic information requires tight spatiotemporal regulation of DNA replication factors. In the licensing step of DNA replication, CDT-1 is loaded onto chromatin to subsequently promote the recruitment of additional replication factors, including CDC-45 and GINS. During the elongation step, the CDC-45/GINS complex moves with the replication fork; however, it is largely unknown how its chromatin association is regulated. Here, we show that the chaperone-like ATPase CDC-48/p97 coordinates degradation of CDT-1 with release of the CDC-45/GINS complex. C. elegans embryos lacking CDC-48 or its cofactors UFD-1/NPL-4 accumulate CDT-1 on mitotic chromatin, indicating a critical role of CDC-48 in CDT-1 turnover. Strikingly, CDC-48(UFD-1/NPL-4)-deficient embryos show persistent chromatin association of CDC-45/GINS, which is a consequence of CDT-1 stabilization. Moreover, our data confirmed a similar regulation in Xenopus egg extracts, emphasizing a conserved coordination of licensing and elongation events during eukaryotic DNA replication by CDC-48/p97.

Copyright © 2011 Elsevier Inc. All rights reserved.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics > Chair Genetics - Univ.-Prof. Dr. Olaf Stemmann
Profile Fields > Advanced Fields > Molecular Biosciences
Research Institutions > Research Centres > Bayreuth Center for Molecular Biosciences - BZMB
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics
Profile Fields
Profile Fields > Advanced Fields
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: Yes
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 26 Mar 2015 08:23
Last Modified: 30 Mar 2015 08:42
URI: https://eref.uni-bayreuth.de/id/eprint/8387