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Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle

Title data

Daub, Henrik ; Olsen, Jesper V. ; Bairlein, Michaela ; Gnad, Florian ; Oppermann, Felix S. ; Korner, Roman ; Greff, Zoltán ; Keri, György ; Stemmann, Olaf ; Mann, Matthias:
Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle.
In: Molecular Cell. Vol. 31 (8 August 2008) Issue 3 . - pp. 438-448.
ISSN 1097-4164
DOI: https://doi.org/10.1016/j.molcel.2008.07.007

Official URL: Volltext

Abstract in another language

Protein kinases are pivotal regulators of cell signaling that modulate each other's functions and activities through site-specific phosphorylation events. These key regulatory modifications have not been studied comprehensively, because low cellular abundance of kinases has resulted in their underrepresentation in previous phosphoproteome studies. Here, we combine kinase-selective affinity purification with quantitative mass spectrometry to analyze the cell-cycle regulation of protein kinases. This proteomics approach enabled us to quantify 219 protein kinases from S and M phase-arrested human cancer cells. We identified more than 1000 phosphorylation sites on protein kinases. Intriguingly, half of all kinase phosphopeptides were upregulated in mitosis. Our data reveal numerous unknown M phase-induced phosphorylation sites on kinases with established mitotic functions. We also find potential phosphorylation networks involving many protein kinases not previously implicated in mitotic progression. These results provide a vastly extended knowledge base for functional studies on kinases and their regulation through site-specific phosphorylation.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics > Chair Genetics - Univ.-Prof. Dr. Olaf Stemmann
Profile Fields > Advanced Fields > Molecular Biosciences
Research Institutions > Research Centres > Bayreuth Center for Molecular Biosciences - BZMB
Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Genetics
Profile Fields
Profile Fields > Advanced Fields
Research Institutions
Research Institutions > Research Centres
Result of work at the UBT: No
DDC Subjects: 500 Science > 570 Life sciences, biology
Date Deposited: 26 Mar 2015 11:06
Last Modified: 26 Mar 2015 11:06
URI: https://eref.uni-bayreuth.de/id/eprint/8540