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Allergic cross-reactivity made visible : solution structure of the major cherry allergen Pru av 1

Title data

Neudecker, Philipp ; Schweimer, Kristian ; Nerkamp, Jörg ; Scheurer, Stephan ; Vieths, Stefan ; Sticht, Heinrich ; Rösch, Paul:
Allergic cross-reactivity made visible : solution structure of the major cherry allergen Pru av 1.
In: The Journal of Biological Chemistry. Vol. 276 (2001) Issue 25 . - pp. 22756-22763.
ISSN 1083-351X
DOI: https://doi.org/10.1074/jbc.M101657200

Abstract in another language

Birch pollinosis is often accompanied by hypersensi- tivity to fruit as a consequence of the cross-reaction of pollen allergen-specific IgE antibodies with homologous food proteins. To provide a basis for examining the cross-reactivity on a structural level, we used hetero- nuclear multidimensional NMR spectroscopy to deter- mine the high-resolution three-dimensional structure of the major cherry allergen, Pru av 1, in solution. Based on a detailed comparison of the virtually identical struc- tures of Pru av 1 and Bet v 1, the major birch pollen allergen, we propose an explanation for a significant aspect of the observed cross-reactivity pattern among the family of allergens under consideration. The large hydrophobic cavity expected to be important for the still unknown physiological function of Bet v 1 is con- served in Pru av 1. Structural homology to a domain of human MLN64 associated with cholesterol transport suggests phytosteroids as putative ligands for Pru av 1. NMR spectroscopy provides experimental evidence that Pru av 1 interacts with phytosteroids, and molecular modeling shows that the hydrophobic cavity is large enough to accommodate two such molecules.

Further data

Item Type: Article in a journal
Refereed: Yes
Institutions of the University: Faculties
Faculties > Faculty of Biology, Chemistry and Earth Sciences
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Former Professors > Chair Biopolymers - Univ.-Prof. Dr. Paul Rösch
Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biopolymers > Lehrstuhl Biopolymere - Apl. Prof. Dr. Birgitta Wöhrl
Profile Fields
Profile Fields > Advanced Fields
Profile Fields > Advanced Fields > Polymer and Colloid Science
Result of work at the UBT: Yes
DDC Subjects: 500 Science
500 Science > 500 Natural sciences
Date Deposited: 20 Mar 2015 08:43
Last Modified: 13 Jun 2019 08:07
URI: https://eref.uni-bayreuth.de/id/eprint/8600