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Hendrich, Julian ; Kara, Hatice ; Schweimer, Kristian ; Jacob, Thessa ; Schneider, Maike ; Baldermann, Susanne ; Wöhrl, Birgitta M.:
Identification of glycosylated flavonoids as new physiological ligands of the hazel allergen Cor a 1 : Complex structures reveal different binding orientation and specificity of mono- and disaccharide derivatives.
In: Food Chemistry: X.
Bd. 29
(2025)
.
- 102711.
ISSN 2590-1575
DOI: https://doi.org/10.1016/j.fochx.2025.102711
Abstract
The global production of hazelnuts is rising, but hazelnut allergies remain common and severe. The PR-10-like allergen Cor a 1, found in hazel pollen and nuts, consists of different isoallergens with over 67 % sequence identity. Cor a 1 binds plant secondary metabolites like the diglycosylated flavonoid Q3O-(Glc)-Gal. Understanding the ligand binding of Cor a 1 isoallergens is crucial to understand their physiological and allergenic properties.
We identified physiological mono- and diglycosylated flavonoids from hazel pollen by UHPLC/QTOF-MS and NMR spectroscopy and investigated their binding to Cor a 1 isoallergens by NMR HSQC experiments and ITC titrations. Screening analyses revealed different binding properties of isoallergens. Whereas the isoallergen Cor a 1.0501 bound only monoglycosylated flavonoids, Cor a 1.0401 also bound diglycosylated flavonoids. Protein/ligand complex structures based on NMR NOESY and computational docking experiments revealed different orientations of mono- vs. diglycosylated flavonoids in the amphiphilic pocket and specific binding of disaccharide derivatives.
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| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Keywords: | Cor a 1 isoallergens; PR-10 proteins; Glycosylated flavonoids; ITC; UHPLC/QTOF-MS/MS; NOESY; HSQC; Docking |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Biochemie IV - Biophysikalische Chemie Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Ehemalige ProfessorInnen > Lehrstuhl Biopolymere - Apl. Prof. Dr. Birgitta Wöhrl Fakultäten > Fakultät für Lebenswissenschaften: Lebensmittel, Ernährung und Gesundheit > Lehrstuhl Food Metabolome > Lehrstuhl Food Metabolome - Univ.-Prof. Dr. Susanne Baldermann |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik > 500 Naturwissenschaften |
| Eingestellt am: | 04 Aug 2025 06:06 |
| Letzte Änderung: | 04 Aug 2025 06:06 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/94395 |