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Electrospun PLLA Nanofiber Scaffolds and Their Use in Combination with BMP-2 for Reconstruction of Bone Defects

Titelangaben

Schofer, Markus D. ; Roessler, Philip P. ; Schaefer, Jan ; Theisen, Christina ; Schlimme, Sonja ; Heverhagen, Johannes T. ; Völker, Maximilian ; Dersch, Roland ; Agarwal, Seema ; Fuchs-Winkelmann, Susanne ; Paletta, Jürgen R. J.:
Electrospun PLLA Nanofiber Scaffolds and Their Use in Combination with BMP-2 for Reconstruction of Bone Defects.
In: PLoS One. Bd. 6 (28 September 2011) Heft 9 .
ISSN 1932-6203
DOI: https://doi.org/10.1371/journal.pone.0025462

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Projektfinanzierung: Deutsche Forschungsgemeinschaft

Abstract

Introduction: Adequate migration and differentiation of mesenchymal stem cells is essential for regeneration of large bone defects. To achieve this, modern graft materials are becoming increasingly important. Among them, electrospun nanofiber scaffolds are a promising approach, because of their high physical porosity and potential to mimic the extracellular matrix (ECM).

Materials and Methods: The objective of the present study was to examine the impact of electrospun PLLA nanofiber scaffolds on bone formation in vivo, using a critical size rat calvarial defect model. In addition we analyzed whether direct incorporation of bone morphogenetic protein 2 (BMP-2) into nanofibers could enhance the osteoinductivity of the scaffolds. Two critical size calvarial defects (5 mm) were created in the parietal bones of adult male Sprague-Dawley rats. Defects were either (1) left unfilled, or treated with (2) bovine spongiosa, (3) PLLA scaffolds alone or (4) PLLA/BMP-2 scaffolds. Cranial CT-scans were taken at fixed intervals in vivo. Specimens obtained after euthanasia were processed for histology, histomorphometry and immunostaining (Osteocalcin, BMP-2 and Smad5).

Results: PLLA scaffolds were well colonized with cells after implantation, but only showed marginal ossification. PLLA/BMP-2 scaffolds showed much better bone regeneration and several ossification foci were observed throughout the defect. PLLA/BMP-2 scaffolds also stimulated significantly faster bone regeneration during the first eight weeks compared to bovine spongiosa. However, no significant differences between these two scaffolds could be observed after twelve weeks. Expression of osteogenic marker proteins in PLLA/BMP-2 scaffolds continuously increased throughout the observation period. After twelve weeks osteocalcin, BMP-2 and Smad5 were all significantly higher in the PLLA/BMP-2 group than in all other groups.

Conclusion: Electrospun PLLA nanofibers facilitate colonization of bone defects, while their use in combination with BMP-2 also increases bone regeneration in vivo and thus combines osteoconductivity of the scaffold with the ability to maintain an adequate osteogenic stimulus.

Weitere Angaben

Publikationsform: Artikel in einer Zeitschrift
Begutachteter Beitrag: Ja
Keywords: MESENCHYMAL STEM-CELLS; PEPTIDE-AMPHIPHILE NANOFIBERS; SIZE CALVARIAL DEFECT; MORPHOGENETIC PROTEIN-2; OSTEOGENIC DIFFERENTIATION; GROWTH-FACTOR; MOLECULAR ARCHITECTURE; HYBRID SCAFFOLD; DUAL DELIVERY; STROMAL CELLS
Institutionen der Universität: Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Makromolekulare Chemie II
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie > Lehrstuhl Makromolekulare Chemie II > Lehrstuhl Makromolekulare Chemie II - Univ.-Prof. Dr. Andreas Greiner
Profilfelder > Advanced Fields > Polymer- und Kolloidforschung
Fakultäten
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften
Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Chemie
Profilfelder
Profilfelder > Advanced Fields
Titel an der UBT entstanden: Nein
Themengebiete aus DDC: 500 Naturwissenschaften und Mathematik > 540 Chemie
500 Naturwissenschaften und Mathematik > 570 Biowissenschaften; Biologie
600 Technik, Medizin, angewandte Wissenschaften > 600 Technik
600 Technik, Medizin, angewandte Wissenschaften > 610 Medizin und Gesundheit
Eingestellt am: 01 Apr 2015 13:30
Letzte Änderung: 01 Apr 2015 13:30
URI: https://eref.uni-bayreuth.de/id/eprint/9802