at Google ScholarTitle data
Acin-Perez, Rebeca ; Russwurm, Michael ; Günnewig, Kathrin ; Gertz, Melanie ; Zoidl, Georg ; Ramos, Lavoisier ; Buck, Jochen ; Levin, Lonny R. ; Rassow, Joachim ; Manfredi, Giovanni ; Steegborn, Clemens:
A phosphodiesterase 2A isoform localized to mitochondria regulates respiration.
In: The Journal of Biological Chemistry.
Vol. 286
(2011)
Issue 35
.
- pp. 30423-30432.
ISSN 1083-351X
DOI: https://doi.org/10.1074/jbc.M111.266379
Abstract in another language
Mitochondria are central organelles in cellular energy metabolism, apoptosis, and aging processes. A signaling network regulating these functions was recently shown to include soluble adenylyl cyclase as a local source of the second messenger cAMP in the mitochondrial matrix. However, a mitochondrial cAMP-degrading phosphodiesterase (PDE) necessary for switching off this cAMP signal has not yet been identified. Here, we describe the identification and characterization of a PDE2A isoform in mitochondria from rodent liver and brain. We find that mitochondrial PDE2A is located in the matrix and that the unique N terminus of PDE2A isoform 2 specifically leads to mitochondrial localization of this isoform. Functional assays show that mitochondrial PDE2A forms a local signaling system with soluble adenylyl cyclase in the matrix, which regulates the activity of the respiratory chain. Our findings complete a cAMP signaling cascade in mitochondria and have implications for understanding the regulation of mitochondrial processes and for their pharmacological modulation.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Additional notes: | PubMed-ID: 9932481 |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry > Chair Biochemistry - Univ.-Prof. Dr. Clemens Steegborn Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science > 540 Chemistry |
| Date Deposited: | 17 Apr 2015 09:27 |
| Last Modified: | 16 Jun 2023 08:50 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/10378 |