at Google ScholarTitle data
Rauh, David ; Fischer, Frank ; Gertz, Melanie ; Lakshminarasimhan, Mahadevan ; Bergbrede, Tim ; Aladini, Firouzeh ; Kambach, Christian ; Becker, Christian F. W. ; Zerweck, Johannes ; Schutkowski, Mike ; Steegborn, Clemens:
An acetylome peptide microarray reveals specificities and deacetylation substrates for all human sirtuin isoforms.
In: Nature Communications.
Vol. 4
(2013)
.
- 2327.
ISSN 2041-1723
DOI: https://doi.org/10.1038/ncomms3327
Abstract in another language
Sirtuin enzymes regulate metabolism and aging processes through deacetylation of acetyl-lysines in target proteins. More than 6,800 mammalian acetylation sites are known, but few targets have been assigned to most sirtuin isoforms, hampering our understanding of sirtuin function. Here we describe a peptide microarray system displaying 6,802 human acetylation sites for the parallel characterisation of their modification by deacetylases. Deacetylation data for all seven human sirtuins obtained with this system reveal isoform-specific substrate preferences and deacetylation substrate candidates for all sirtuin isoforms, including Sirt4. We confirm malate dehydrogenase protein as a Sirt3 substrate and show that peroxiredoxin 1 and high-mobility group B1 protein are deacetylated by Sirt5 and Sirt1, respectively, at the identified sites, rendering them likely new in vivo substrates. Our microarray platform enables parallel studies on physiological acetylation sites and the deacetylation data presented provide an exciting resource for the identification of novel substrates for all human sirtuins.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Additional notes: | PubMed-ID: 23995836 |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry I - Proteinbiochemie der Signaltransduktion > Chair Biochemistry - Univ.-Prof. Dr. Clemens Steegborn Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Chemistry > Chair Biochemistry I - Proteinbiochemie der Signaltransduktion |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science > 540 Chemistry |
| Date Deposited: | 20 Apr 2015 12:50 |
| Last Modified: | 09 Jan 2025 09:48 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/10424 |