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Sauheitl, Leopold ; Glaser, Bruno ; Weigelt, Alexandra:
Advantages of compound-specific stable isotope measurements over bulk measurements in studies on plant uptake of intact amino acids.
In: Rapid Communications in Mass Spectrometry.
Bd. 23
(2009)
Heft 20
.
- S. 3333-3342.
ISSN 0951-4198
DOI: https://doi.org/10.1002/rcm.4255
Abstract
Increasing interest in the ability of plants to take up amino acids has given rise to questions on theaccuracy of the commonly used bulk method to measure and calculate amino acid uptake. Thismethod uses bulk measurements of 13C and 15N enrichment in plant tissues after application of duallabelledamino acids but some authors have recommended the use of compound-specific stableisotope (CSI) analysis of the plants’ amino acids instead. However, there has never been a directevaluation of both methods.We conducted a field study applying dual-labelled (13C, 15N) amino acids(glycine, valine, tyrosine and lysine) to soil of a Plantago lanceolata monoculture. Root and shootsamples were collected 24 h after label application and the isotope composition of the plant tissueswas investigated using bulk and CSI measurements. Enrichment of 13C in the case of CSI measurementswas limited to the applied amino acids, showing that no additional 13C had been incorporatedinto the plants’ amino acid pool via the uptake of tracer-derived C-fragments. Compared with thisrather conservative indicator of amino acid uptake, the 13C enrichment of bulk measurements was 8,5, 1.6 and 6 times higher for fine roots, storage roots, shoot and the whole plant, respectively. Thesefindings show that the additional uptake of tracer-derived C-fragments will result in a considerableoverestimation of amino acid uptake in the case of bulk measurements. We therefore highlyrecommend the use of CSI measurements for future amino acid uptake studies due to their higheraccuracy. Copyright # 2009 John Wiley & Sons, Ltd.
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| Publikationsform: | Artikel in einer Zeitschrift |
|---|---|
| Begutachteter Beitrag: | Ja |
| Zusätzliche Informationen: | BAYCEER76513 |
| Institutionen der Universität: | Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Geowissenschaften > Lehrstuhl Bodenphysik Forschungseinrichtungen > Forschungszentren > Bayreuther Zentrum für Ökologie und Umweltforschung - BayCEER Fakultäten Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften Fakultäten > Fakultät für Biologie, Chemie und Geowissenschaften > Fachgruppe Geowissenschaften Forschungseinrichtungen Forschungseinrichtungen > Forschungszentren |
| Titel an der UBT entstanden: | Ja |
| Themengebiete aus DDC: | 500 Naturwissenschaften und Mathematik |
| Eingestellt am: | 06 Jul 2015 10:25 |
| Letzte Änderung: | 06 Jul 2015 10:25 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/15908 |