at Google ScholarTitle data
Liu, Ya-Jun ; Liu, Shuang-Jiang ; Drake, Harold L. ; Horn, Marcus A.:
Consumers of 4‐chloro‐2‐methylphenoxyacetic acid from agricultural soil and drilosphere harbor cadA, r/sdpA, and tfdA‐like gene encoding oxygenases.
In: FEMS Microbiology Ecology.
Vol. 86
(2013)
Issue 1
.
- pp. 114-129.
ISSN 1574-6941
DOI: https://doi.org/10.1111/1574-6941.12144
Abstract in another language
Microbial degradation of 2-Methyl-4-chlorophenoxyacetic acid (MCPA) in soil is enhanced by earthworms and intiated by tfdA-like, cadA and r/sdpA gene encoded oxygenases. Copy numbers of such genes increased during MCPA degradation in soil, and MCPA stimulated transcription of tfdA-like and r/sdpA genes up to 4x. Transcription of cadA was detected with MCPA only. DNA stable isotope probing after consumption of 0.6-0.8 mg 13C-MCPA gdw-1 in oxic microcosms indicated diverse labeled oxygenase genes in bulk soil, burrow walls and cast. 9, 6 and 3 operational taxonomic units of tfdA-like, cadA and r/sdpA genes, respectively, were labeled, and affiliated with group 2 Alphaproteobacteria including Bradyrhizobia and group 1 class III Betaproteobacteria. New genes encoding putative MCPA oxygenases were identified. Diversity of labeled OTUs tended to be lower for cast than for bulk soil. The collective data indicate (i) hitherto unknown active MCPA degraders and/or oxygenase genes in soil, (ii) that multiple oxygenases are associated with MCPA degradation in soil at the same time, (iii) that earthworms impact the capability of MCPA degraders in soil to respond to MCPA, and (iv) enables a more in depth analysis of MCPA degrader communities in soil by future structural gene based experimental strategies.
Further data
| Item Type: | Article in a journal |
|---|---|
| Refereed: | Yes |
| Additional notes: | BAYCEER115399 |
| Institutions of the University: | Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology > Chair Ecological Microbiology Research Institutions > Research Centres > Bayreuth Center of Ecology and Environmental Research- BayCEER Faculties Faculties > Faculty of Biology, Chemistry and Earth Sciences Faculties > Faculty of Biology, Chemistry and Earth Sciences > Department of Biology Research Institutions Research Institutions > Research Centres |
| Result of work at the UBT: | Yes |
| DDC Subjects: | 500 Science |
| Date Deposited: | 22 Jul 2015 05:41 |
| Last Modified: | 21 Jul 2021 06:15 |
| URI: | https://eref.uni-bayreuth.de/id/eprint/16982 |